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Testing Data (Staining of frozen mouse olfactory bulb usingMBS611723. Image courtesy of Carl Olson, PhD, and James Nagy, PhD, University of Manitoba, Canada.)

Rabbit anti-Human, Mouse Connexin 36 Polyclonal Antibody | anti-CX36 antibody

Connexin 36 (CX36) (HRP)

Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
Connexin 36; Polyclonal Antibody; Connexin 36 (CX36) (HRP); anti-CX36 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Connexin 36 on transfected HeLa cell lysates, mouse brain, retina and pancreas homogenates. The specificity of this antibody was demonstrated using Connexin 36 knockout mice.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-CX36 antibody
Immunohistochemistry (IHC) Frozen, ELISA (EIA), Western Blot (WB)
Application Notes
IHC-F: 2-5ug/ml
ELISA: 0.1-1ug/ml
WB: 1-3ug/ml; detects a single band at ~36kD.
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the C-terminal region of the human Connexin 36 protein.
Conjugate
HRP
Positive Control
Mouse Connexin 36 transfected HeLa cell lysates, mouse brain, retina and pancreas homogenates.
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Testing Data

(Staining of frozen mouse olfactory bulb usingMBS611723. Image courtesy of Carl Olson, PhD, and James Nagy, PhD, University of Manitoba, Canada.)

Testing Data (Staining of frozen mouse olfactory bulb usingMBS611723. Image courtesy of Carl Olson, PhD, and James Nagy, PhD, University of Manitoba, Canada.)

Testing Data

(Staining of mouse pancreatic islet cells usingMBS611723. Image courtesy of Carl Olson, PhD, and James Nagy, PhD, University of Manitoba, Canada.)

Testing Data (Staining of mouse pancreatic islet cells usingMBS611723. Image courtesy of Carl Olson, PhD, and James Nagy, PhD, University of Manitoba, Canada.)
Product Categories/Family for anti-CX36 antibody
References
1. Bennett, M.V., et al., Neuron 6(3): 305-320 (1991). 2. Condorelli, D.F., et al., Eur. J. Neurosci. 10(3): 1202-1208 (1998). 3. Kumar, N.M. & Gilula, N.B., Cell 84(3): 381-388 (1996). 4. Kuraoka, A., et al., J. Histochem. Cytochem. 41(7): 971-980 (1993). 5. Rosental, R., et al., Brain Res. Brain Res. Rev. 32(1): 11-15 (2000). 6. Sohl, G., et al., FEBS Lett 428(1-2): 27-31 (1998). 7. Sohl, G., et al., Eur. J. Cell Biol. 69(3): 267-275 (1996). 8. Srinivas, M., et al., J. Neurosci. 19(22): 9848-9855 (1999). 9. Wilgenbus, K.K., et al., Int. J. Cancer 51(4): 522-529 (1992).

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Product Notes

The CX36 (Catalog #AAA6470105) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Connexin 36 (CX36) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 36 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen, ELISA (EIA), Western Blot (WB). IHC-F: 2-5ug/ml ELISA: 0.1-1ug/ml WB: 1-3ug/ml; detects a single band at ~36kD. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CX36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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