Rabbit COMT Polyclonal Antibody | anti-COMT antibody

Rabbit Polyclonal to Human COMT

Cat. Num. AAA244727

• Gene Names: COMT; HEL-S-98n
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: COMT; Polyclonal Antibody; Rabbit Polyclonal to Human COMT; Anti-COMT Antibody (N-Terminus) IHC-plus; Catechol O-methyltransferase; Catechol-O-methyltransferase; Human COMT; anti-COMT antibody

• Host: Rabbit
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human COMT. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 221

• Applicable Applications for anti-COMT antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 19 amino acid peptide from N-terminus of human COMT. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%); Marmoset (89%); Hamster (84%).
• Immunogen Type: Synthetic peptide
• Immunogen: COMT antibody was raised against synthetic 19 amino acid peptide from N-terminus of human COMT. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%); Marmoset (89%); Hamster (84%).
• Antigen Modification: N-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-COMT antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-COMT antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-COMT antibody

Catechol O-methyltransferase (COMT) is involved in the inactivation of catecholamines, including the neurotransmitter dopamine. A Val(108/158) Met functional polymorphism of the COMT gene has been shown to affect working memory-associated frontal lobe function in humans.

NCBI and Uniprot Product Information

• NCBI GI #: 6466450
• NCBI GeneID: 1312
• NCBI Accession #: NP_009294.1
• NCBI GenBank Nucleotide #: NM_007310.2
• UniProt Accession #: P21964; Q6IB07; Q6ICE6; Q9BWC7; A8MPV9
• Molecular Weight: 24,449 Da
• NCBI Official Full Name: catechol O-methyltransferase isoform S-COMT
• NCBI Official Synonym Full Names: catechol-O-methyltransferase
• NCBI Official Symbol: COMT
• NCBI Official Synonym Symbols: HEL-S-98n
• NCBI Protein Information: catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n
• UniProt Protein Name: Catechol O-methyltransferase
• Protein Family: Catechol O-methyltransferase
• UniProt Gene Name: COMT
• UniProt Entry Name: COMT_HUMAN

NCBI Description

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

Uniprot Description

COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 2.1.1.6; Membrane protein, integral; Methyltransferase; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: postsynaptic membrane; mitochondrion; membrane; axon; plasma membrane; integral to membrane; dendritic spine; cytosol

Molecular Function: protein binding; magnesium ion binding; catechol O-methyltransferase activity; O-methyltransferase activity

Biological Process: methylation; response to drug; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to lipopolysaccharide; response to pain; female pregnancy; learning; reproductive process in a multicellular organism; response to organic cyclic substance; positive regulation of homocysteine metabolic process; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process

Disease: Schizophrenia; Panic Disorder 1