Human Prokineticin Receptor 2 (PKR2) ELISA Kit | PKR2 elisa kit

Human Prokineticin Receptor 2 (PKR2) ELISA Kit

Cat. Num. AAA2022341

• Gene Names: PROKR2; HH3; KAL3; PKR2; GPRg2; GPR73b; GPR73L1; dJ680N4.3
• Reactivity: Human
• Synonyms: Prokineticin Receptor 2 (PKR2); Human Prokineticin Receptor 2 (PKR2) ELISA Kit; PROKR2; KAL3; GPR73L1; GPR73b; GPRg2; G Protein-Coupled receptor 73-Like 1; PKR2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prokineticin Receptor 2 (PKR2).; No significant cross-reactivity or interference between Prokineticin Receptor 2 (PKR2) and analogues was observed.
• Sequence Length: 384

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.107ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prokineticin Receptor 2 (PKR2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prokineticin Receptor 2 (PKR2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKR2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prokineticin Receptor 2 (PKR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prokineticin Receptor 2 (PKR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prokineticin Receptor 2 (PKR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prokineticin Receptor 2 (PKR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PKR2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21426829
• NCBI GeneID: 128674
• NCBI Accession #: NP_658986.1
• NCBI GenBank Nucleotide #: NM_144773.2
• UniProt Accession #: Q8NFJ6; Q2M3C0; Q5TDY1; Q9NTT0; A5JUU1
• Molecular Weight: 43,996 Da
• NCBI Official Full Name: prokineticin receptor 2
• NCBI Official Synonym Full Names: prokineticin receptor 2
• NCBI Official Symbol: PROKR2
• NCBI Official Synonym Symbols: HH3; KAL3; PKR2; GPRg2; GPR73b; GPR73L1; dJ680N4.3
• NCBI Protein Information: prokineticin receptor 2
• UniProt Protein Name: Prokineticin receptor 2
• UniProt Gene Name: PROKR2
• UniProt Synonym Gene Names: GPR73L1; PKR2; PK-R2
• UniProt Entry Name: PKR2_HUMAN

NCBI Description

Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor for prokineticins. The encoded protein is similar in sequence to GPR73, another G protein-coupled receptor for prokineticins. [provided by RefSeq, Jul 2008]

Uniprot Description

PROKR2: Receptor for prokineticin 2. Exclusively coupled to the G(q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. Defects in PROKR2 are the cause of Kallmann syndrome type 3 (KAL3); also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin- releasing hormone-synthesizing neurons. KAL3 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip and/or palate, selective tooth agenesis, and bimanual synkinesis. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.3

Cellular Component: integral to membrane; plasma membrane

Molecular Function: neuropeptide Y receptor activity

Biological Process: circadian rhythm; neuropeptide signaling pathway

Disease: Hypogonadotropic Hypogonadism 3 With Or Without Anosmia