Goat Complement C3, C3c Polyclonal Antibody | anti-C3 antibody

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, AHUS5, ARMD9, C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1, Complement Component 3, Complement Factor 3, CPAMD1) (FITC)

Cat. Num. AAA6003284

• Reactivity: The antiSerum does not cross-react with any other component of Rabbit plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunocytochemistry
• Purity: Purified
• Synonyms: Complement C3; C3c; Polyclonal Antibody; C3c (Complement Component C3c; C3/C3c; Acylation Stimulating Protein Cleavage Product; ASP; AHUS5; ARMD9; C3 and PZP-like alpha-2-macroglobulin Domain-containing Protein 1; Complement Component 3; Complement Factor 3; CPAMD1) (FITC); Goat anti Rabbit Complement C3; C3c (ASP; anti-C3 antibody

• Host: Goat
• Reactivity: The antiSerum does not cross-react with any other component of Rabbit plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to C3c fragment of Rabbit complement factor C3.
• Purity/Purification: Purified
• Form/Format: FITC; Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

• Applicable Applications for anti-C3 antibody: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Other applications not tested.
• Application Notes: Recommended Dilution:; Immunohistochemistry: Frozen; ; Optimal dilutions to be determined by the researcher.
• Source: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Product: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
• Preparation and Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Related Product Information for anti-C3 antibody

In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of rabbit serum or plasma. The fluorescent immunoconjugate to rabbit C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the identification can be based on directly on laboratory findings. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Product Categories/Family for anti-C3 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 99028969
• NCBI GeneID: 280677
• NCBI Accession #: NP_001035559.2
• NCBI GenBank Nucleotide #: NM_001040469.2
• UniProt Accession #: Q2UVX4; Q2KIZ4
• NCBI Official Full Name: complement C3 preproprotein
• NCBI Official Synonym Full Names: complement component 3<
• NCBI Official Symbol: C3
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: ASP
• UniProt Entry Name: CO3_BOVIN

Uniprot Description

Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates

By similarity.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes

By similarity.Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2

By similarity.

Subunit structure: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). C3dg interacts with CR2 (via the N-terminal Sushi domains 1 and 2). Interacts with VSIG4. Interacts (both C3a and ASP) with C5AR2; the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of C5AR2, recruitment of ARRB2 to the cell surface and endocytosis of GRP77

By similarity. Interacts with BHV-1 glycoprotein C. Ref.3

Subcellular location: Secreted.

Post-translational modification: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons

By similarity.Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.