Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) RTU ELISA Kit | SLPI rtu elisa kit

Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

Cat. Num. AAA4502546

• Gene Names: Slpi; ALP
• Reactivity: Mouse
• Synonyms: Secretory Leukocyte Peptidase Inhibitor (SLPI); Mouse Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit; ALK1; ALP; MPI; BLPI; HUSI; HUSI-I; WAP4; WFDC4; Antileukoproteinase; Seminal proteinase inhibitor; Mucus proteinase inhibitor; WAP four-disulfide core domain protein 4; SLPI rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 131

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 35pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLPI rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLPI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SLPI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6755574
• NCBI GeneID: 20568
• NCBI Accession #: NP_035544.1
• NCBI GenBank Nucleotide #: NM_011414.3
• UniProt Accession #: P97430; O09081; O09082
• Molecular Weight: 14,308 Da
• NCBI Official Full Name: antileukoproteinase
• NCBI Official Synonym Full Names: secretory leukocyte peptidase inhibitor
• NCBI Official Symbol: Slpi
• NCBI Official Synonym Symbols: ALP
• NCBI Protein Information: antileukoproteinase
• UniProt Protein Name: Antileukoproteinase
• Protein Family: Antileukoproteinase
• UniProt Gene Name: Slpi
• UniProt Synonym Gene Names: ALP

Uniprot Description

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:9126337). Modulates the innate immune response after bacterial infection (PubMed:12615907). Contributes to regulate the inflammatory and immune responses to the intracellular parasite L.major (PubMed:25030421). Down-regulates responses to bacterial lipopolysaccharide (LPS) (PubMed:9039268, PubMed:12615907, PubMed:25030421). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:11017147, PubMed:12615907). Has antimicrobial activity against mycobacteria, but not against salmonella (PubMed:18322212). Contributes to normal resistance against infection by M.tuberculosis (PubMed:18322212). Required for normal resistance to L.major (PubMed:25030421). Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (PubMed:11017147, PubMed:25030421). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells ().