Testing Data
(Figure 1: Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney)
Rabbit Collagen IV Polyclonal Antibody
Rabbit anti Collagen IV
Reactivity
Bovine, Human, Rat, Swine
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
Collagen IV; Polyclonal Antibody; Rabbit anti Collagen IV; anti-Collagen IV antibody
Host
Rabbit
Reactivity
Bovine, Human, Rat, Swine
Clonality
Polyclonal
Specificity
Polyclonal type IV collagen recognizes the extracellular - basement membranes.
Form/Format
Each vial contains 250ul polyclonal Serum including 0.09% sodium azide
Applicable Applications for anti-Collagen IV antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room tempeRature. Optimal antibody dilution should be determined by titRation; 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
This polyclonal type IV collagen antibody is a Rabbit polyclonal antibody. The Rabbits were immunized with isolated Human type IV collagen. The immunogen has been isolated from Human placenta.
Preparation and Storage
Store at 4 degree C, or in small aliquots for prolonged storage at -20 degree C.
Testing Data
(Figure 1: Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney)
Testing Data
(Figure 1: Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney)
Flow Cytometry (FC/FACS)
(Figure 2: Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney )
Flow Cytometry (FC/FACS)
(Figure 2: Collagen type IV staining of basement membranes and connective tissue in a frozen section of human kidney )
Related Product Information for anti-Collagen IV antibody
The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasma. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiSerum is important in the differential identification of bullous lesions in dermatopathology.
References
Visser, R., van der Beek, J.M.H., Havenith, M.G., Cleutjens, J.P.M. and Bosman, F.T. (1985). Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia. Histopathology 10, 171-180. Cleutjens, J.P.M., Havenith, M.G., Kasper, M., Vallinga, M., and Bosman, F.T. (1990). Absence of type IV collagen in the centre of the corneal epithelial basement membrane. Histochemical Journal 22, 688-94. Havenith, M.G. et al. (1987). Human specific anti-type IV collagen monoclonal antibodies, characterisation and immunohistochemical appliCation. Histochemistry 87, 123-28. Schapers, R.F.M., Pauwels, R.P.E., Havenith, M.G., Smeets, A.W.G.B, van den Brandt and P.A. Bosman, F.T. (1990). Prognostic Significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder. Cancer 66, 2583-88.
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Product Notes
The Collagen IV (Catalog #AAA573001) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Collagen IV reacts with Bovine, Human, Rat, Swine and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen IV can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room tempeRature. Optimal antibody dilution should be determined by titRation; 1:25 - 1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the Collagen IV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen IV, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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