Western Blot (WB)
(COL8A2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).)
Rabbit anti-Human COL8A2 Polyclonal Antibody | anti-COL8A2 antibody
COL8A2 Antibody (C-term)
Western Blot (WB)
(COL8A2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).)
Western Blot (WB)
(COL8A2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).)
Immunohistochemistry (IHC)
(COL8A2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL8A2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(COL8A2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL8A2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of COL8A2 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
The protein is a major component of the basement membrane of the
corneal endothelium and forms homo- or heterotrimers with alpha 1
(VIII) type collagens. Defects in this gene are associated with
Fuchs endothelial corneal dystrophy and posterior polymorphous
corneal dystrophy type 2.
Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007)
Aldave, A.J., et al. Cornea 26(8):963-965(2007)
Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006) :
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.
Protein type: Extracellular matrix; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 1p34.2
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane
Molecular Function: protein binding, bridging; extracellular matrix structural constituent
Biological Process: collagen catabolic process; extracellular matrix disassembly; cell-cell adhesion; epithelial cell proliferation; extracellular matrix organization and biogenesis; camera-type eye morphogenesis; angiogenesis
Disease: Corneal Dystrophy, Posterior Polymorphous, 2; Corneal Dystrophy, Fuchs Endothelial, 1
Research Articles on COL8A2
Similar Products
Product Notes
The COL8A2 col8a2 (Catalog #AAA9207971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 571-599. The COL8A2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL8A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the COL8A2 col8a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL8A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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