Rabbit anti-Human COL4A6 Polyclonal Antibody | anti-COL4A6 antibody

COL4A6, ID (COL4A6, Collagen alpha-6(IV) chain)

Cat. Num. AAA646422

• Gene Names: COL4A6; DELXq22.3; CXDELq22.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: COL4A6; Polyclonal Antibody; ID (COL4A6; Collagen alpha-6(IV) chain); Anti -COL4A6; anti-COL4A6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COL4A6.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-COL4A6 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide corresponding to a region between aa828-858 from the central region of human COL4A6, conjugated to KLH. Cellular Localization: secreted, extracellular space, extracellular matrix, basement membrane.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(COL4A6 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the COL4A6 antibody detected the COL4A6 protein (arrow).)

Related Product Information for anti-COL4A6 antibody

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Two splice variants have been identified for this gene.

Product Categories/Family for anti-COL4A6 antibody

Antibodies; Abs to Collagen

NCBI and Uniprot Product Information

• NCBI GI #: 148536823
• NCBI GeneID: 1288
• NCBI Accession #: NP_001838.2
• NCBI GenBank Nucleotide #: NM_001847.2
• UniProt Accession #: Q14031; Q12823; Q14053; Q5JYH6; Q5JYH8; Q9NQM5; Q9NTX3; Q9UJ76; Q9UMG6; Q9Y4L4
• Molecular Weight: 163,807 Da
• NCBI Official Full Name: collagen alpha-6(IV) chain isoform A
• NCBI Official Synonym Full Names: collagen, type IV, alpha 6
• NCBI Official Symbol: COL4A6
• NCBI Official Synonym Symbols: DELXq22.3; CXDELq22.3
• NCBI Protein Information: collagen alpha-6(IV) chain; collagen alpha-6(IV) chain; collagen IV, alpha-6 polypeptide; dJ889N15.4 (Collagen Alpha 6(IV)); collagen of basement membrane, alpha-6
• UniProt Protein Name: Collagen alpha-6(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A6
• UniProt Entry Name: CO4A6_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Subunit structure: There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network.

Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane.

Domain: Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

Post-translational modification: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues

By similarity.

Involvement in disease: Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Ref.6 Ref.7

Sequence similarities: Belongs to the type IV collagen family.Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.