Rabbit anti-Human COL4A6 Polyclonal Antibody | anti-COL4A6 antibody

COL4A6, ID (COL4A6, Collagen alpha-6(IV) chain) (PE)

Cat. Num. AAA6287723

• Gene Names: COL4A6; DELXq22.3; CXDELq22.3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COL4A6; Polyclonal Antibody; ID (COL4A6; Collagen alpha-6(IV) chain) (PE); Collagen alpha-6(IV) chain; anti-COL4A6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COL4A6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-COL4A6 antibody: Western Blot (WB)
• Application Notes: WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between a portion within aa828-858 from the central region of human COL4A6, conjugated to KLH. Cellular Localization: secreted, extracellular space, extracellular matrix, basement membrane.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COL4A6 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the COL4A6 antibody detected the COL4A6 protein (arrow).)

Product Categories/Family for anti-COL4A6 antibody

Antibodies; Collagen

References

1. Romero, R., et al. Am. J. Obstet. Gynecol. (2010). In press: 2. Bailey, S.D., et al. Diabetes Care (2010). In press: 3. Romero, R., et al. Am. J. Obstet. Gynecol. 202 (5), 431 (2010). 4. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 5. Skovbjerg, H., et al. BMC Cancer 9, 136 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 148536823
• NCBI GeneID: 1288
• NCBI Accession #: NP_001838.2; NP_378667.1
• NCBI GenBank Nucleotide #: NM_001847.2
• UniProt Accession #: Q14031; Q12823; Q14053; Q5JYH6; Q5JYH8; Q9NQM5; Q9NTX3; Q9UJ76; Q9UMG6; Q9Y4L4
• Molecular Weight: 163,807 Da
• NCBI Official Full Name: collagen alpha-6(IV) chain isoform A
• NCBI Official Synonym Full Names: collagen, type IV, alpha 6
• NCBI Official Symbol: COL4A6
• NCBI Official Synonym Symbols: DELXq22.3; CXDELq22.3
• NCBI Protein Information: collagen alpha-6(IV) chain; collagen alpha-6(IV) chain; collagen IV, alpha-6 polypeptide; dJ889N15.4 (Collagen Alpha 6(IV)); collagen of basement membrane, alpha-6
• UniProt Protein Name: Collagen alpha-6(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A6
• UniProt Entry Name: CO4A6_HUMAN

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL4A6: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell adhesion

Disease: Deafness, X-linked 6