Testing Data
phospho-Cofilin 1 (Ser-3) Peptide
phospho-Cofilin 1 (Ser-3) Blocking Peptide
Synonyms
phospho-Cofilin 1 (Ser-3) Peptide; phospho-Cofilin 1 (Ser-3) Blocking Peptide; phospho-Cofilin 1 (Ser-3) Peptide peptide
Specificity
The peptide is specifically recognized by Cofilin 1 (Ser-3) phospho-specific antibody (CP1151) in ELISA, and has been shown to block the reactivity of CP1151 during Western blot. In addition, the peptide is recommended for use in blocking CP1151 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-Cofilin 1 (Ser-3) synthetic peptide corresponds to amino acid residues around serine 3 of human Cofilin 1. This peptide sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid differences from human Cofilin 2.
Application Notes
Antibody Blocking: 1: 1000
ELISA: 50 ng/well
ELISA: 50 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for phospho-Cofilin 1 (Ser-3) Peptide peptide
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (Cofilin 2), non-muscle cofilin (Cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
References
• Bamburg, J.R. (1999). Annu Rev Cell Dev Biol. 15:185
• Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007
• Maciver, S.K. & Hussey, P.J. (2002). Genome Biol. 3(5): 3007
Similar Products
Product Notes
The phospho-Cofilin 1 (Ser-3) Peptide (Catalog #AAA474028) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Antibody Blocking: 1: 1000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the phospho-Cofilin 1 (Ser-3) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-Co filin 1 (Ser-3) synthetic peptide correspond s to amino acid residues around serine 3 of human Cofilin 1. This peptide sequence has 100% homology with similar regions of rat and mouse Cofilin 1, and has two amino acid difference s from human Cofilin 2. It is sometimes possible for the material contained within the vial of "phospho-Cofilin 1 (Ser-3) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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