Western Blot (WB)
((0.3ug/ml) staining of Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
Goat FSTL1 Polyclonal Antibody | anti-FSTL1 antibody
Goat anti-FSTL1 Antibody
Gene Names
FSTL1; FRP; FSL1; MIR198
Reactivity
Tested: Human; Expected from sequence similarity: Human, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
FSTL1; Polyclonal Antibody; Goat anti-FSTL1 Antibody; follistatin-like 1; FRP; FSL1; follistatin-related protein; FLJ50214; FLJ52277; FSTL1 antibody; follistatin-like 1 antibody; FRP antibody; FSL1 antibody; follistatin-related protein antibody; FLJ50214 antibody; FLJ52277 antibody; anti-FSTL1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TAEKTKRVSTKEI
Sequence Length
308
Applicable Applications for anti-FSTL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Experiments gave bands at approx 55kDa and 40kDa in Human Placenta lysate after 0.3ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Tanaka et al, Int Immunol.1998 Sep;10(9): 1305-14.; PMID: 9786430). This protein has a calculated MW of 35. 0kDa according to NP_009016.1. Recommended concentration: 0.3-1ug/ml.
Western Blot: Experiments gave bands at approx 55kDa and 40kDa in Human Placenta lysate after 0.3ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Tanaka et al, Int Immunol.1998 Sep;10(9): 1305-14.; PMID: 9786430). This protein has a calculated MW of 35. 0kDa according to NP_009016.1. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-TAEKTKRVSTKEI, from the C Terminus of the protein sequence according to NP_009016.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.3ug/ml) staining of Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,228 Da
NCBI Official Full Name
follistatin-related protein 1
NCBI Official Synonym Full Names
follistatin like 1
NCBI Official Symbol
FSTL1
NCBI Official Synonym Symbols
FRP; FSL1; MIR198
NCBI Protein Information
follistatin-related protein 1
UniProt Protein Name
Follistatin-related protein 1
Protein Family
UniProt Gene Name
FSTL1
UniProt Synonym Gene Names
FRP
UniProt Entry Name
FSTL1_HUMAN
NCBI Description
This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: extracellular region; extracellular space
Molecular Function: calcium ion binding; heparin binding; protein binding
Biological Process: BMP signaling pathway; response to starvation
Research Articles on FSTL1
Similar Products
Product Notes
The FSTL1 fstl1 (Catalog #AAA421364) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-FSTL1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Experiments gave bands at approx 55kDa and 40kDa in Human Placenta lysate after 0.3ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Tanaka et al, Int Immunol.1998 Sep;10(9): 1305-14.; PMID: 9786430). This protein has a calculated MW of 35. 0kDa according to NP_009016.1. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the FSTL1 fstl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TAEKTKRVST KEI. It is sometimes possible for the material contained within the vial of "FSTL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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