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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Cocaine And Amphetamine Regulated Transcript Polyclonal Antibody | anti-CART antibody

Polyclonal Antibody to Cocaine And Amphetamine Regulated Transcript (CART)

Gene Names
Cartpt; Cart
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Cocaine And Amphetamine Regulated Transcript; Polyclonal Antibody; Polyclonal Antibody to Cocaine And Amphetamine Regulated Transcript (CART); anti-CART antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CART. It has beenselected for its ability to recognize CART in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
116
Applicable Applications for anti-CART antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
CART (Gln28~Leu129)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant CART (Gln28~Leu129) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041294
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Adrenal gland Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Adrenal gland Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,696 Da
NCBI Official Full Name
cocaine- and amphetamine-regulated transcript protein
NCBI Official Synonym Full Names
CART prepropeptide
NCBI Official Symbol
Cartpt
NCBI Official Synonym Symbols
Cart
NCBI Protein Information
cocaine- and amphetamine-regulated transcript protein
UniProt Protein Name
Cocaine- and amphetamine-regulated transcript protein
Protein Family
UniProt Gene Name
Cartpt
UniProt Synonym Gene Names
Cart

NCBI Description

neuronal protein that may play a role in brain development and may be regulated by testosterone [RGD, Feb 2006]

Uniprot Description

CARTPT: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro. Belongs to the CART family.

Protein type: Hormone; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: extracellular space; secretory granule

Molecular Function: neuropeptide hormone activity; protein binding

Biological Process: activation of MAPKK activity; adult feeding behavior; cell glucose homeostasis; cellular response to starvation; circadian regulation of gene expression; G-protein coupled receptor signaling pathway; gastric acid secretion; negative regulation of appetite; negative regulation of bone resorption; neuropeptide signaling pathway; positive regulation of blood pressure; positive regulation of epinephrine secretion; positive regulation of transmission of nerve impulse; regulation of bone remodeling; regulation of insulin secretion; synaptic transmission

Research Articles on CART

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Product Notes

The CART cartpt (Catalog #AAA2026203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cocaine And Amphetamine Regulated Transcript (CART) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cocaine And Amphetamine Regulated Transcript can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the CART cartpt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cocaine And Amphetamine Regulated Transcript, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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