Rabbit anti-Human, Mouse NELFB Polyclonal Antibody | anti-NELFB antibody

NELFB Antibody

Cat. Num. AAA9429772

• Gene Names: NELFB; COBRA1; NELF-B
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: NELFB; Polyclonal Antibody; NELFB Antibody; COBRA1; NELF-B; anti-NELFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The antibody detects endogenous levels of total NELFB protein.
• Purity/Purification: Antigen affinity purification
• Form/Format: In pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
• Concentration: 1.6mg/ml (varies by lot)
• Sequence Length: 628

• Applicable Applications for anti-NELFB antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1: 25-100
• Immunogen: Synthetic peptide corresponding to internal residues of human NELFB
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 46625(NELFB Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200))

Related Product Information for anti-NELFB antibody

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008.

Product Categories/Family for anti-NELFB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 862669351
• NCBI GeneID: 25920
• NCBI Accession #: NP_056271
• NCBI GenBank Nucleotide #: NM_015456.4
• UniProt Accession #: Q8WX92; Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3
• Molecular Weight: 65,697 Da
• NCBI Official Full Name: negative elongation factor B
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B
• NCBI Protein Information: negative elongation factor B
• UniProt Protein Name: Negative elongation factor B
• UniProt Gene Name: NELFB
• UniProt Synonym Gene Names: COBRA1; KIAA1182; NELF-B

NCBI Description

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Uniprot Description

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.