Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse F8A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Coagulation Factor VIII Associated Protein 1 (F8A1) Polyclonal Antibody | anti-F8A1 antibody
Polyclonal Antibody to Coagulation Factor VIII Associated Protein 1 (F8A1)
Gene Names
F8a; F8a1; DXUcsf1; AI852759
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Coagulation Factor VIII Associated Protein 1 (F8A1); Polyclonal Antibody; Polyclonal Antibody to Coagulation Factor VIII Associated Protein 1 (F8A1); anti-F8A1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against F8A1. It has been selected for its ability to recognize F8A1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HLPLMPLAAL QALGDAASCQ LLARDYTGAL ALFTRMQRLA REHGGHPVQQ LELLPQPPSG PQPPLSGPQP RPVLGSTLPL PQPPDHAPGS VAPSPGTLGA FADVLVRCEV SRVLLLLLLQ PPPAKLLPEH AQTLEKYSWE AFDGHGQDTS GQLPEELFLL LQSLVMAAQE KDTEGIKKLQ VEMWPLLTAE QNHLLHLVLQ
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HLPLMPLAAL QALGDAASCQ LLARDYTGAL ALFTRMQRLA REHGGHPVQQ LELLPQPPSG PQPPLSGPQP RPVLGSTLPL PQPPDHAPGS VAPSPGTLGA FADVLVRCEV SRVLLLLLLQ PPPAKLLPEH AQTLEKYSWE AFDGHGQDTS GQLPEELFLL LQSLVMAAQE KDTEGIKKLQ VEMWPLLTAE QNHLLHLVLQ
Sequence Length
2351
Applicable Applications for anti-F8A1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant F8A1 (His171~Pro375) expressed in E.coli.
Cross Reactivity
Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038838
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse F8A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml Rabbit Anti-Mouse F8A1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
40,490 Da
NCBI Official Full Name
Factor VIII intron 22 protein
NCBI Official Synonym Full Names
factor 8-associated gene A
NCBI Official Symbol
F8a
NCBI Official Synonym Symbols
F8a1; DXUcsf1; AI852759
NCBI Protein Information
factor VIII intron 22 protein
UniProt Protein Name
Factor VIII intron 22 protein
Protein Family
UniProt Gene Name
F8a1
UniProt Synonym Gene Names
F8a
Uniprot Description
F8A1: Not known. Possible housekeeping role.
Chromosomal Location of Human Ortholog: X A7.3|X 37.29 cM
Cellular Component: cytoplasm; nucleus
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Product Notes
The F8A1 f8a1 (Catalog #AAA2015440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Coagulation Factor VIII Associated Protein 1 (F8A1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Coagulation Factor VIII Associated Protein 1 (F8A1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the F8A1 f8a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- HLPLMPLAAL QALGDAASCQ LLARDYTGAL ALFTRMQRLA REHGGHPVQQ LELLPQPPSG PQPPLSGPQP RPVLGSTLPL PQPPDHAPGS VAPSPGTLGA FADVLVRCEV SRVLLLLLLQ PPPAKLLPEH AQTLEKYSWE AFDGHGQDTS GQLPEELFLL LQSLVMAAQE KDTEGIKKLQ VEMWPLLTAE QNHLLHLVLQ. It is sometimes possible for the material contained within the vial of "Coagulation Factor VIII Associated Protein 1 (F8A1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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