Mouse ALDH1L1 Monoclonal Antibody | anti-ALDH1L1 antibody

ALDH1L1 antibody

Cat. Num. AAA831369

• Gene Names: ALDH1L1; FDH; FTHFD; 10-fTHF; 10-FTHFDH
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: ALDH1L1 antibody was purified by affinity chromatography.
• Synonyms: ALDH1L1; Monoclonal Antibody; ALDH1L1 antibody; Monoclonal ALDH1L1; Anti-ALDH1L1; Aldehyde Dehydrogenase 1 Family Member L1; DKFZp781N0997; FTHFD; ALDHL 1; ALDHL-1; anti-ALDH1L1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2A6
• Purity/Purification: ALDH1L1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 902

• Applicable Applications for anti-ALDH1L1 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:500-2000
• Biological Significance: ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.
• Cross-Reactivity: Human, Dog, Rat, Monkey
• Immunogen: ALDH1L1 antibody was raised in mouse using a full length recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALDH1L1 protein (Right) or empty vector (Left) detected with ALDH1L1 antibody)

Related Product Information for anti-ALDH1L1 antibody

Mouse monoclonal ALDH1L1 antibody

Product Categories/Family for anti-ALDH1L1 antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 21614513
• NCBI GeneID: 10840
• NCBI Accession #: NP_036322
• NCBI GenBank Nucleotide #: NM_012190.3
• UniProt Accession #: O75891; Q68CS1; Q8TBP8; B4DG36; E9PBX3
• Molecular Weight: 55,394 Da
• NCBI Official Full Name: cytosolic 10-formyltetrahydrofolate dehydrogenase isoform 2
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member L1
• NCBI Official Symbol: ALDH1L1
• NCBI Official Synonym Symbols: FDH; FTHFD; 10-fTHF; 10-FTHFDH
• NCBI Protein Information: cytosolic 10-formyltetrahydrofolate dehydrogenase
• UniProt Protein Name: Cytosolic 10-formyltetrahydrofolate dehydrogenase
• Protein Family: Cytosolic 10-formyltetrahydrofolate dehydrogenase
• UniProt Gene Name: ALDH1L1
• UniProt Synonym Gene Names: FTHFD; 10-FTHFDH; FDH
• UniProt Entry Name: AL1L1_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

ALDH1L1: 2 isoforms of the human protein are produced by alternative splicing

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.6; Oxidoreductase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: mitochondrion

Molecular Function: methyltransferase activity; aldehyde dehydrogenase (NAD) activity; hydroxymethyl-, formyl- and related transferase activity; formyltetrahydrofolate dehydrogenase activity; catalytic activity

Biological Process: methylation; biosynthetic process; one-carbon compound metabolic process; 10-formyltetrahydrofolate catabolic process