Western Blot (WB)
(Western blot analysis of extracts of human testis tissue, using CNOT10 antibody.)
Rabbit anti-Human, Mouse CNOT10 Polyclonal Antibody | anti-CNOT10 antibody
CNOT10 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CNOT10; Polyclonal Antibody; CNOT10 Antibody; CCR4 NOT transcription complex subunit 10; CCR4-NOT transcription complex subunit 10; CNOT 10; CNOTA_HUMAN; DKFZp434K115; FLJ12890; FLJ13165; anti-CNOT10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CNOT10 antibody detects endogenous levels of CNOT10.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
717
Applicable Applications for anti-CNOT10 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human CNOT10.
Subcellular Location
Nucleus
Predicted Cross Reactivity
Horse, Rabbit, Dog, Chicken
Similarity
Horse (100%), Rabbit (83%), Dog (100%), Chicken (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of human testis tissue, using CNOT10 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of human testis tissue, using CNOT10 antibody.)
Immunohistochemistry (IHC)
(MBS9603594 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603594 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CNOT10 antibody
Function: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.
Subunit Structure: Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. CNOT10 and CNOT11 form a subcomplex docked to the CNOT1 scaffold.
Similarity: Belongs to the CNOT10 family.
Subunit Structure: Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. CNOT10 and CNOT11 form a subcomplex docked to the CNOT1 scaffold.
Similarity: Belongs to the CNOT10 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 82 kDa
Predicted: 83 kDa
Predicted: 83 kDa
NCBI Official Full Name
CCR4-NOT transcription complex subunit 10 isoform 2
NCBI Official Synonym Full Names
CCR4-NOT transcription complex subunit 10
NCBI Official Symbol
CNOT10
NCBI Protein Information
CCR4-NOT transcription complex subunit 10
UniProt Protein Name
CCR4-NOT transcription complex subunit 10
Protein Family
UniProt Gene Name
CNOT10
Uniprot Description
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.
Research Articles on CNOT10
Similar Products
Product Notes
The CNOT10 cnot10 (Catalog #AAA9603594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNOT10 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CNOT10 cnot10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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