Rabbit anti-Human cMyc Polyclonal Antibody | anti-cMyc antibody

c-Myc (C10) Antibody

Cat. Num. AAA440055

• Gene Names: MYC; MRTL; MYCC; c-Myc; bHLHe39
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: cMyc; Polyclonal Antibody; c-Myc (C10) Antibody; anti-cMyc antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human c-Myc p67, recognizes the 9E10 Myc tag
• Form/Format: 200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.200 mg/ml (varies by lot)
• Sequence Length: 60

• Applicable Applications for anti-cMyc antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Recommended starting dilution for western bloting 1:200.
• Origin: c-Myc (C10) is provided as an affinity purified rabbit polyclonal antibody, raised against a synthetic peptide mapping near the carboxy terminal domain of human c-Myc.
• Immunogen: synthetic peptide mapping near the carboxy terminal domain of human c-Myc.
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-cMyc antibody

The transcription factor c-Myc is a proto-oncogene that is at the focal point in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis (1-3). In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated (2&3).c-Myc also plays a pivotal role in apoptosis, most notably its connections to the CD95/Fas death receptor pathway (1&4). These different biological responses to c-Myc are most likely the result of different overlapping subsets of c-Myc target genes (1).

References

1.) Yakisich JS, Sandoval PY, Morrison TL, Kapler GM. 2006. TIF1 activates the intra-S-phase checkpoint response in the diploid micronucleus and amitotic polyploid macronucleus of Tetrahymena. Mol Biol Cell. Dec;17(12):5185-97.2.) Schmauch C, Maniak M. 2008. Competition between targeting signals in hybrid proteins provides information on their relative in vivo affinities for subcellular compartments. Eur J Cell Biol. Feb;87(2):57-68.

1. Hoffman B, Amanullah A, Shafarenko M, Liebermann DA. 2002. The proto-oncogene c-myc in hematopoietic development and leukemogenesis. Oncogene 21(21): 3414-3421.2. Boxer LM, Dang CV. 2001. Translocations involving c-myc and c-myc function. Oncogene 20(40):5595-5610.3. Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K. 1999. Function of the c-Myc oncogenic transcription factor. Exp Cell Res 253(1): 63-77.4. Prendergast GC. 1999. Mechanisms of apoptosis by c-Myc. Oncogene 18(19):2967-2987.

NCBI and Uniprot Product Information

• NCBI GI #: 396512
• NCBI GeneID: 4609
• UniProt Accession #: Q6LBK7; P01106
• Molecular Weight: 7,012 Da
• NCBI Official Full Name: c-myc, partial
• NCBI Official Synonym Full Names: v-myc avian myelocytomatosis viral oncogene homolog
• NCBI Official Symbol: MYC
• NCBI Official Synonym Symbols: MRTL; MYCC; c-Myc; bHLHe39
• NCBI Protein Information: myc proto-oncogene protein; avian myelocytomatosis viral oncogene homolog; class E basic helix-loop-helix protein 39; myc-related translation/localization regulatory factor; proto-oncogene c-Myc; transcription factor p64; v-myc myelocytomatosis viral oncogene homolog
• UniProt Protein Name: C-myc protein
• UniProt Gene Name: c-myc
• UniProt Entry Name: Q6LBK7_HUMAN

NCBI Description

The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.

Protein type: Nucleolus; Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: cellular iron ion homeostasis; oxygen transport; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; regulation of telomere maintenance; transcription, DNA-dependent; MAPKKK cascade; negative regulation of cell division; negative regulation of monocyte differentiation; negative regulation of stress-activated MAPK cascade; chromatin remodeling; ureteric bud branching; negative regulation of fibroblast proliferation; regulation of gene expression; energy reserve metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

Disease: Burkitt Lymphoma