Rabbit CLTB Polyclonal Antibody | anti-CLTB antibody

CLTB Antibody

Cat. Num. AAA9611651

• Gene Names: CLTB; LCB
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CLTB; Polyclonal Antibody; CLTB Antibody; anti-CLTB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CLTB Antibody detects endogenous levels of CLTB.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-CLTB antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human CLTB.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using CLTB Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain tissue, using CLTB Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-CLTB antibody

Description: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Subunit Structure: Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R.

Similarity: Belongs to the clathrin light chain family.

NCBI and Uniprot Product Information

• NCBI GI #: 4502901
• NCBI GeneID: 1212
• NCBI Accession #: NP_001825.1
• NCBI GenBank Nucleotide #: NP_001825.1
• UniProt Accession #: P09497
• Molecular Weight: Observed: 32 kDa; Predicted: 26 kDa
• NCBI Official Full Name: clathrin light chain B isoform a
• NCBI Official Synonym Full Names: clathrin light chain B
• NCBI Official Symbol: CLTB
• NCBI Official Synonym Symbols: LCB
• NCBI Protein Information: clathrin light chain B
• UniProt Protein Name: Clathrin light chain B
• Protein Family: Clathrin
• UniProt Gene Name: CLTB
• UniProt Synonym Gene Names: Lcb
• UniProt Entry Name: CLCB_HUMAN

NCBI Description

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CLTB: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: clathrin coat of trans-Golgi network vesicle; clathrin coat; intracellular membrane-bound organelle; cytoplasm; plasma membrane; trans-Golgi network; clathrin coat of coated pit

Molecular Function: protein binding; structural molecule activity; peptide binding

Biological Process: vesicle-mediated transport; intracellular protein transport