Rabbit anti-Human CLTA Polyclonal Antibody | anti-CLTA antibody

CLTA, ID (Clathrin Light Chain A, Lca) (AP)

Cat. Num. AAA6469197

• Gene Names: CLTA; LCA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CLTA; Polyclonal Antibody; ID (Clathrin Light Chain A; Lca) (AP); anti-CLTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLTA.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CLTA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CLTA (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLTA Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CLTA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of CLTA (arrow) using rabbit polyclonal CLTA Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene.)

Flow Cytometry (FC/FACS)

(CLTA Antibody (Center) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-CLTA antibody

Antibodies; Clathrin

References

1. Wong, D.H., et.al., J. Neurosci. 10(9), 3025-3031 (1990). 2. Nathke, I., et.al., J. Biol. Chem. 265(30), 18621-18627 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 4502899
• NCBI GeneID: 1211
• NCBI Accession #: NP_001824.1; NP_009027.1; NP_001070145.1; NP_001825.1; NP_009028.1
• NCBI GenBank Nucleotide #: NM_001833.3
• UniProt Accession #: P09496; Q2XPN5; Q53XZ1; A8K4W3; B4DIN1; F5H6N3
• Molecular Weight: 17,687 Da
• NCBI Official Full Name: clathrin light chain A isoform a
• NCBI Official Synonym Full Names: clathrin light chain A
• NCBI Official Symbol: CLTA
• NCBI Official Synonym Symbols: LCA
• NCBI Protein Information: clathrin light chain A
• UniProt Protein Name: Clathrin light chain A
• Protein Family: Clathrin
• UniProt Gene Name: CLTA
• UniProt Synonym Gene Names: Lca
• UniProt Entry Name: CLCA_HUMAN

NCBI Description

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]

Uniprot Description

CLTA: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Belongs to the clathrin light chain family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: clathrin coat of trans-Golgi network vesicle; clathrin coat; intracellular membrane-bound organelle; membrane; cytoplasmic membrane-bound vesicle; plasma membrane; trans-Golgi network membrane; cytosol; clathrin coat of coated pit

Molecular Function: protein binding; clathrin heavy chain binding; structural molecule activity; peptide binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; intracellular protein transport; nerve growth factor receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; post-Golgi vesicle-mediated transport