Typical Testing Data/Standard Curve (for reference only)
Human PAPPA2 ELISA Kit | PAPPA2 elisa kit
Human PAPPA2 (Pappalysin 2) ELISA Kit
Gene Names
PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2
Reactivity
Human
Synonyms
PAPPA2; Human PAPPA2 (Pappalysin 2) ELISA Kit; PAPPA2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PAPPA2. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PAPPA2 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
1791
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAPPA2 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAPPA2 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAPPA2. You can calculate the concentration of Human PAPPA2 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAPPA2. You can calculate the concentration of Human PAPPA2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,136 Da
NCBI Official Full Name
pappalysin-2 isoform 1
NCBI Official Synonym Full Names
pappalysin 2
NCBI Official Symbol
PAPPA2
NCBI Official Synonym Symbols
PAPPE; PLAC3; PAPP-E; PAPP-A2
NCBI Protein Information
pappalysin-2; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; pregnancy-associated plasma protein E1
UniProt Protein Name
Pappalysin-2
Protein Family
UniProt Gene Name
PAPPA2
UniProt Synonym Gene Names
PLAC3; PAPP-A2; PAPP-E
UniProt Entry Name
PAPP2_HUMAN
NCBI Description
This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
Uniprot Description
PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; EC 3.4.24.-; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q25.2
Cellular Component: membrane; extracellular region; intracellular
Molecular Function: metallopeptidase activity; zinc ion binding
Biological Process: cellular protein metabolic process; regulation of cell growth; cell differentiation; proteolysis
Research Articles on PAPPA2
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Product Notes
The Human PAPPA2 pappa2 (Catalog #AAA2500517) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500517 ELISA Kit recognizes Human PAPPA2. It is sometimes possible for the material contained within the vial of "PAPPA2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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