Rabbit anti-Human CLOCK Polyclonal Antibody

CLOCK, ID (Circadian Locomoter Output Cycles Protein Kaput, hCLOCK, Class E Basic Helix-loop-helix Protein 8, BHLHE8, KIAA0334)

Cat. Num. AAA628395

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CLOCK; Polyclonal Antibody; ID (Circadian Locomoter Output Cycles Protein Kaput; hCLOCK; Class E Basic Helix-loop-helix Protein 8; BHLHE8; KIAA0334); Anti -CLOCK; anti-CLOCK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLOCK.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CLOCK antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the Center region of human CLOCK (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CLOCK Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). CLOCK (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CLOCK antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-CLOCK antibody

CLOCK belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within this protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.

Product Categories/Family for anti-CLOCK antibody

Antibodies; Abs to Circadian Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6478192
• NCBI Official Full Name: clock
• Protein Family: Clock-controlled protein

Uniprot Description

CLOCK: ARNTL/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL. Plays a role in DNA damage response (DDR) signaling during the S phase. Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif. Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).

Protein type: DNA-binding; EC 2.3.1.48; Acetyltransferase; Transcription factor

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: nucleoplasm; transcription factor complex; intracellular membrane-bound organelle; chromosome; nucleus; cytosol

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; histone acetyltransferase activity; DNA binding; chromatin DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; proteasomal ubiquitin-dependent protein catabolic process; establishment and/or maintenance of chromatin architecture; positive regulation of transcription, DNA-dependent; response to redox state; signal transduction; activation of NF-kappaB transcription factor; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; photoperiodism; DNA damage checkpoint; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; histone acetylation; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; regulation of insulin secretion; regulation of hair cycle; positive regulation of inflammatory response