Chicken growth hormone, GH ELISA Kit | GH elisa kit

Chicken growth hormone, GH ELISA Kit

Cat. Num. AAA703063

• Gene Names: GH1; GH; GHN; GH-N; hGH-N; IGHD1B
• Reactivity: Chicken
• Synonyms: growth hormone; GH; Chicken growth hormone; GH ELISA Kit; GH ELISA KIT; GH elisa kit

• Reactivity: Chicken
• Sequence Length: 217

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 625pg/ml-10000pg/ml
• Sensitivity: 312.5 pg/ml
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GH elisa kit

Introduction: Growth hormone is a protein hormone of about 190 amino acids that is synthesized and secreted by cells called somatotrophs in the anterior pituitary. It is a major participant in control of several complex physiologic processes, including growth and metabolism. Growth hormone is also of considerable interest as a drug used in both humans and animals. Growth is a very complex process, and requires the coordinated action of several hormones. The major role of growth hormone in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-I. IGF-I stimulates proliferation of chondrocytes (cartilage cells), resulting in bone growth. Growth hormone does seem to have a direct effect on bone growth in stimulating differentiation of chondrocytes. IGF-I also appears to be the key player in muscle growth. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth hormone has important effects on protein, lipid and carbohydrate metabolism. In some cases, a direct effect of growth hormone has been clearly demonstrated, in others, IGF-I is thought to be the critical mediator, and some cases it appears that both direct and indirect effects are at play.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to GH has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated GH and incubated. A competitive inhibition reaction is launched between GH (Standards or samples) and Biotin-conjugated GH with the pre-coated antibody specific for GH. The more amount of GH in samples, the less antibody bound by Biotin-conjugated GH. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of GH in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 183157
• NCBI GeneID: 2688
• UniProt Accession #: P01241; Q14405; Q16631; Q5EB53; Q9HBZ1; Q9UMJ7; Q9UNL5; A6NEF6
• Molecular Weight: 20,201 Da
• NCBI Official Full Name: growth hormone
• NCBI Official Synonym Full Names: growth hormone 1
• NCBI Official Symbol: GH1
• NCBI Official Synonym Symbols: GH; GHN; GH-N; hGH-N; IGHD1B
• NCBI Protein Information: somatotropin; pituitary growth hormone
• UniProt Protein Name: Somatotropin
• Protein Family: Envelope glycoprotein
• UniProt Gene Name: GH1
• UniProt Synonym Gene Names: GH; GH-N
• UniProt Entry Name: SOMA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.