Rabbit anti-Human CLIC6 Polyclonal Antibody | anti-CLIC6 antibody

CLIC6, ID (CLIC6, CLIC1L, Chloride intracellular channel protein 6, Parchorin) (Azide free) (HRP)

Cat. Num. AAA6286760

• Gene Names: CLIC6; CLIC1L
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CLIC6; Polyclonal Antibody; ID (CLIC6; CLIC1L; Chloride intracellular channel protein 6; Parchorin) (Azide free) (HRP); Parchorin; anti-CLIC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLIC6.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CLIC6 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 539-565 in the central region of human CLIC6. Species sequence homology: mouse, rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6008261. This demonstrates that MBS6008261 detected the CLIC6 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue tissue using MBS6008261 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008261 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-CLIC6 antibody

Antibodies; Ion Channel

References

1. Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009). 2. Friedli, M., et al. Gene 320, 31-40 (2003). 3. Strippoli, P., et al. Mamm. Genome 13(8):456-462(2002). 4. Scanlan, M.J., et al. Cancer Immun. 1, 4 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 27894378
• NCBI GeneID: 54102
• NCBI Accession #: NP_444507.1
• NCBI GenBank Nucleotide #: NM_053277.1
• UniProt Accession #: Q96NY7; Q8IX31; A8K0U8
• Molecular Weight: 73,012 Da
• NCBI Official Full Name: chloride intracellular channel protein 6
• NCBI Official Synonym Full Names: chloride intracellular channel 6
• NCBI Official Symbol: CLIC6
• NCBI Official Synonym Symbols: CLIC1L
• NCBI Protein Information: chloride intracellular channel protein 6; parchorin; chloride channel form A
• UniProt Protein Name: Chloride intracellular channel protein 6
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC6
• UniProt Synonym Gene Names: CLIC1L
• UniProt Entry Name: CLIC6_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel family of proteins. The gene is part of a large triplicated region found on chromosomes 1, 6, and 21. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CLIC6: May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.12

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein C-terminus binding; chloride channel activity; protein homodimerization activity; voltage-gated ion channel activity; D4 dopamine receptor binding; D3 dopamine receptor binding; D2 dopamine receptor binding