Western Blot (WB)
(Western Blot (WB) analysis of CLIC1 polyclonal antibody)
Rabbit CLIC1 Polyclonal Antibody | anti-CLIC1 antibody
CLIC1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CLIC1; Polyclonal Antibody; CLIC1 Polyclonal Antibody; Chloride intracellular channel protein 1; Chloride channel ABP; Nuclear chloride ion channel 27; NCC27; Regulatory nuclear chloride ion channel protein; hRNCC; G6; anti-CLIC1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CLIC1 polyclonal antibody detects endogenous levels of CLIC1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
241
Applicable Applications for anti-CLIC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:10-1:100
IHC: 1:50-1:200
IF: 1:10-1:100
Immunogen
Recombinant full length Human CLIC1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CLIC1 polyclonal antibody)
Western Blot (WB)
(Western Blot (WB) analysis of CLIC1 polyclonal antibody)
Related Product Information for anti-CLIC1 antibody
Chloride intracellular channel 1 (CLIC1), also referred to as NCC27, is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. CLIC1 is a monomeric protein that contains a redox-active site similar to glutaredoxin; it functions as a anionselective channel. CLIC1 forms a dimer when oxidized and is then able to form chloride ion channels in bilayers and vesicles, whereas a reducing environment prevents this from occurring. Insulin concentration also plays a role in CLIC1 regulation, and the hormone may cause a subnuclear relocalization of CLIC1. CLIC1 is associated with macrophage activation; a downregulation of CLIC1 function prevents TNFalpha release induced by beta-Amyloid protein (A-beta) stimulation. This suggests a role for CLIC1 in several neurodegenerative processes, such as Alzheimer's disease, a syndrome characterized by an accumulation of A-beta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 27kDa
NCBI Official Full Name
chloride intracellular channel protein 1
UniProt Protein Name
Chloride intracellular channel protein 1
Protein Family
UniProt Gene Name
CLIC1
UniProt Synonym Gene Names
G6; NCC27; NCC27; hRNCC
UniProt Entry Name
CLIC1_HUMAN
Uniprot Description
CLIC1: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Belongs to the chloride channel CLIC family.
Protein type: Membrane protein, integral; Channel, chloride
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: extracellular space; nuclear membrane; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nuclear envelope; nucleus; vesicle; brush border
Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity
Biological Process: positive regulation of osteoblast differentiation; regulation of mitochondrial membrane potential; regulation of cell cycle; chloride transport; signal transduction
Similar Products
Product Notes
The CLIC1 clic1 (Catalog #AAA3011523) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLIC1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CLIC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the CLIC1 clic1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLIC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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