CLIC1 recombinant protein

CLIC1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121834

• Gene Names: CLIC1; G6; NCC27
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CLIC1; CLIC1 Protein; Human; Recombinant (His Tag); Human CLIC1 Protein (His Tag); G6 Protein; NCC27 Protein; chloride intracellular channel 1; CLIC1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys241

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human CLIC1 (O00299)(Met1-Lys241) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CLIC1 recombinant protein

Background: Members of the CLIC family are largely soluble proteins that possess the intriguing property of spontaneous insertion into phospholipid bilayers to form integral membrane ion channels. Chloride intracellular channel 1 (CLIC1), a newly discovered member of the chloride channel protein family, has been implicated in multiple human cancers. CLIC1 is a Chloride Intracellular Ion Channel protein that exists either in a soluble state in the cytoplasm or as a membrane bound protein. CLIC1 acts as a putative oncogene in pancreatic cancer and may represent a novel test and therapeutic target for pancreatic cancer. Intracellular chloride channel protein 1 (CLIC1) participates in inflammatory processes by regulating macrophage phagosomal functions such as pH and proteolysis. CLIC1 is a novel therapeutic target to help reduce the adaptive immune response in autoimmune diseases. The expression of CLIC1 might be closely related to the carcinogenesis, clinical biological behaviors, and prognosis of pancreatic ductal adenocarcinomas. Intracellular chloride channel 1 (CLIC1), a novel metamorphic protein, acts as a sensor of cell oxidation and is involved in inflammation.

NCBI and Uniprot Product Information

• NCBI GI #: 567757607
• NCBI GeneID: 1192
• NCBI Accession #: NP_001274522.1
• NCBI GenBank Nucleotide #: NM_001287593.1
• UniProt Accession #: O00299; Q15089; Q502X1
• Molecular Weight: 26,923 Da
• NCBI Official Full Name: chloride intracellular channel protein 1
• NCBI Official Synonym Full Names: chloride intracellular channel 1
• NCBI Official Symbol: CLIC1
• NCBI Official Synonym Symbols: G6; NCC27
• NCBI Protein Information: chloride intracellular channel protein 1; RNCC protein; chloride channel ABP; hRNCC; nuclear chloride ion channel 27; nuclear chloride ion channel protein; p64CLCP; regulatory nuclear chloride ion channel protein
• UniProt Protein Name: Chloride intracellular channel protein 1
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC1
• UniProt Synonym Gene Names: G6; NCC27; NCC27; hRNCC
• UniProt Entry Name: CLIC1_HUMAN

NCBI Description

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]

Uniprot Description

CLIC1: Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle. Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral; Channel, chloride

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; nuclear membrane; mitochondrion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nuclear envelope; nucleus; vesicle; brush border

Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity

Biological Process: positive regulation of osteoblast differentiation; regulation of mitochondrial membrane potential; regulation of cell cycle; chloride transport; signal transduction