Rabbit anti-Human, Mouse ZC3H8 Polyclonal Antibody | anti-ZC3H8 antibody

ZC3H8, NT (ZC3H8, ZC3HDC8, Zinc finger CCCH domain-containing protein 8) (PE)

Cat. Num. AAA6364852

• Gene Names: ZC3H8; Fliz1; ZC3HDC8
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZC3H8; Polyclonal Antibody; NT (ZC3H8; ZC3HDC8; Zinc finger CCCH domain-containing protein 8) (PE); Zinc finger CCCH domain-containing protein 8; anti-ZC3H8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ZC3H8 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ZC3H8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-90 amino acids from the N-terminal region of human ZC3H8.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZC3H8 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ZC3H8 antibody detected the ZC3H8 protein (arrow).)

Flow Cytometry (FC/FACS)

(ZC3H8 Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ZC3H8 antibody

Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Product Categories/Family for anti-ZC3H8 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Lamesch, P., et al. Genomics 89(3):307-315(2007). Hwang, E.S., et al. Immunology 106(4):464-469(2002). Hwang, E.S., et al. J. Immunol. 169(1):248-253(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 217416362
• NCBI GeneID: 84524
• NCBI Accession #: NP_115883.2
• NCBI GenBank Nucleotide #: NM_032494.2
• UniProt Accession #: Q8N5P1; Q9BZ75
• Molecular Weight: 33,576 Da
• NCBI Official Full Name: zinc finger CCCH domain-containing protein 8
• NCBI Official Synonym Full Names: zinc finger CCCH-type containing 8
• NCBI Official Symbol: ZC3H8
• NCBI Official Synonym Symbols: Fliz1; ZC3HDC8
• NCBI Protein Information: zinc finger CCCH domain-containing protein 8; zinc finger CCCH type domain containing 8; zinc finger CCCH-type domain containing 8
• UniProt Protein Name: Zinc finger CCCH domain-containing protein 8
• Protein Family: Zinc finger CCCH domain-containing protein
• UniProt Gene Name: ZC3H8
• UniProt Synonym Gene Names: ZC3HDC8
• UniProt Entry Name: ZC3H8_HUMAN

Uniprot Description

ZC3H8: Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 2q13

Cellular Component: Cajal body; transcription elongation factor complex; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity

Biological Process: response to antibiotic; apoptosis; snRNA transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; T cell homeostasis; negative regulation of T cell differentiation in the thymus; negative regulation of transcription, DNA-dependent; snRNA transcription from RNA polymerase III promoter