Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human CLIC1 Polyclonal Antibody | anti-CLIC1 antibody
CLIC1 (Chloride Intracellular Channel 1, cDNA, FLJ94671, Homo Sapiens Chloride Intracellular Channel 1 (CLIC1), mRNA, DADB-110M10.1-001, DAMA-236L13.3-001, DAMC-117B11.2-001, DAQB-350E15.4-001) (MaxLight 750)
Gene Names
CLIC1; G6; CL1C1; NCC27
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
CLIC1; Polyclonal Antibody; CLIC1 (Chloride Intracellular Channel 1; cDNA; FLJ94671; Homo Sapiens Chloride Intracellular Channel 1 (CLIC1); mRNA; DADB-110M10.1-001; DAMA-236L13.3-001; DAMC-117B11.2-001; DAQB-350E15.4-001) (MaxLight 750); anti-CLIC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CLIC1.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-CLIC1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.
Immunogen
Recombinant human CLIC1 protein.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CLIC1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane). CLIC1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-CLIC1 Antibody in A375 cell line lysates (35ug/lane). CLIC1(arrow) was detected using the purified Pab.)
Product Categories/Family for anti-CLIC1 antibody
References
1. Singh, H., FEBS J. 274(24), 6306-6316 (2007). 2. Ulmasov, B., (er) BMC Cell Biol. 8, 8 (2007). 3. Edwards, J.C., J. Membr. Biol. 213(1), 39-46 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29.0 kDa (261aa), confirmed by MALDI-TOF
NCBI Official Full Name
chloride intracellular channel protein 1
NCBI Official Synonym Full Names
chloride intracellular channel 1
NCBI Official Symbol
CLIC1
NCBI Official Synonym Symbols
G6; CL1C1; NCC27
NCBI Protein Information
chloride intracellular channel protein 1
Protein Family
NCBI Description
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]
Research Articles on CLIC1
Similar Products
Product Notes
The CLIC1 (Catalog #AAA6468796) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLIC1 (Chloride Intracellular Channel 1, cDNA, FLJ94671, Homo Sapiens Chloride Intracellular Channel 1 (CLIC1), mRNA, DADB-110M10.1-001, DAMA-236L13.3-001, DAMC-117B11.2-001, DAQB-350E15.4-001) (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLIC1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-1:50 FLISA: 1:1,000 WB: 1:50-1:100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CLIC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLIC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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