Typical Testing Data/Standard Curve (for reference only)
Mouse C-type lectin domain family 9 member A (CLEC9A) ELISA Kit | CLEC9A elisa kit
Mouse C-type lectin domain family 9 member A (CLEC9A) ELISA Kit
Gene Names
CLEC9A; DNGR1; UNQ9341
Reactivity
Mouse
Synonyms
C-type lectin domain family 9 member A (CLEC9A); Mouse C-type lectin domain family 9 member A (CLEC9A) ELISA Kit; CLEC9A elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CLEC9A elisa kit
Intended Uses: This CLEC9A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLEC9A. This ELISA kit for research use only!
Principle of the Assay: CLEC9A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC9A antibody and an CLEC9A-HRP conjugate. The assay sample and buffer are incubated together with CLEC9A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC9A concentration since CLEC9A from samples and CLEC9A-HRP conjugate compete for the anti-CLEC9A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC9A from the sample, fewer sites are left to bind CLEC9A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC9A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CLEC9A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC9A antibody and an CLEC9A-HRP conjugate. The assay sample and buffer are incubated together with CLEC9A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC9A concentration since CLEC9A from samples and CLEC9A-HRP conjugate compete for the anti-CLEC9A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC9A from the sample, fewer sites are left to bind CLEC9A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC9A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC9A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,324 Da
NCBI Official Full Name
C-type lectin domain family 9 member A
NCBI Official Synonym Full Names
C-type lectin domain family 9, member A
NCBI Official Symbol
CLEC9A
NCBI Official Synonym Symbols
DNGR1; UNQ9341
NCBI Protein Information
C-type lectin domain family 9 member A; HEEE9341
UniProt Protein Name
C-type lectin domain family 9 member A
Protein Family
UniProt Gene Name
CLEC9A
UniProt Synonym Gene Names
UNQ9341/PRO34046
UniProt Entry Name
CLC9A_HUMAN
NCBI Description
CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells (Huysamen et al., 2008 [PubMed 18408006]).[supplied by OMIM, Aug 2008]
Uniprot Description
CLEC9A: Functions as an endocytic receptor on a small subset of myeloid cells and induces intracellular signaling via spleen tyrosine kinase (Syk). Acts as a receptor for necrotic cells and can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.2
Cellular Component: cell surface; integral to membrane
Molecular Function: carbohydrate binding
Biological Process: receptor-mediated endocytosis; positive regulation of cytokine secretion
Research Articles on CLEC9A
Similar Products
Product Notes
The Mouse CLEC9A clec9a (Catalog #AAA7201465) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7201465 ELISA Kit recognizes Mouse CLEC9A. It is sometimes possible for the material contained within the vial of "C-type lectin domain family 9 member A (CLEC9A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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