Rabbit Cleaved-MMP-14 Polyclonal Antibody | anti-MMP-14 antibody

Cleaved-MMP-14 (Y112) Polyclonal Antibody

Cat. Num. AAA3008446

• Gene Names: MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cleaved-MMP-14; Polyclonal Antibody; Cleaved-MMP-14 (Y112) Polyclonal Antibody; Matrix metalloproteinase-14; MMP 14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP14; anti-MMP-14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cleaved-MMP-14 (Y112) polyclonal antibody detects endogenous levels of Pro-MMP-14 (65 kDa) or Cleaved-MMP-14 (53 kDa) protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 582

• Applicable Applications for anti-MMP-14 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human MMP-14.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cleaved-MMP-14 (Y112) pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Uterus tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug))

Related Product Information for anti-MMP-14 antibody

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.

NCBI and Uniprot Product Information

• NCBI GI #: 4826834
• NCBI GeneID: 4323
• NCBI Accession #: NP_004986.1
• NCBI GenBank Nucleotide #: NP_004986.1
• UniProt Accession #: P50281
• Molecular Weight: ~ 53, 65kDa
• NCBI Official Full Name: matrix metalloproteinase-14 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 14
• NCBI Official Symbol: MMP14
• NCBI Official Synonym Symbols: MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
• NCBI Protein Information: matrix metalloproteinase-14
• UniProt Protein Name: Matrix metalloproteinase-14
• UniProt Gene Name: MMP14
• UniProt Synonym Gene Names: MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
• UniProt Entry Name: MMP14_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.

Protein type: Protease; EC 3.4.24.80; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; melanosome; plasma membrane

Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity

Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; male gonad development; negative regulation of focal adhesion formation; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; branching morphogenesis of a tube; regulation of transcription, DNA-dependent; ovarian follicle development; response to mechanical stimulus; response to estrogen stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; angiogenesis; response to oxidative stress; astrocyte cell migration; lung development; endochondral ossification; positive regulation of cell migration

Disease: Winchester Syndrome