Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.)
Mouse Clcn3 Monoclonal Antibody | anti-Clcn3 antibody
Clcn3 Antibody: ATTO 390
Gene Names
Clcn3; ClC-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Synonyms
Clcn3; Monoclonal Antibody; Clcn3 Antibody: ATTO 390; CIC3; CLC3; CLCN 3; Chloride channel 3; Chloride channel protein 3; Chloride channel; voltage-sensitive 3; Chloride transporter ClC 3; DKFZp564I0463; H(+)/Cl(-) exchange transporter 3; Chloride transporter ClC-3; ClC-3; anti-Clcn3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S258-5
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
818
Applicable Applications for anti-Clcn3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
1ug/mL (WB), 0.1-1ug/mL (IHC)
Conjugate
ATTO 390
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.)
Related Product Information for anti-Clcn3 antibody
Background Info: Detects ~90kDa. Does not cross-react with Clcn4 or Clcn5 (based on KO validation results)
Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.
Scientific Background: Clcn3 mediates the exchange of chloride ions against protons, and functions as an antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could also help neuronal cells to establish short-term memory.
Product Categories/Family for anti-Clcn3 antibody
References
1. www.phosphosite.org/proteinAction.do?id=5088&showAllSites=true
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,461 Da
NCBI Official Full Name
H(+)/Cl(-) exchange transporter 3
NCBI Official Synonym Full Names
chloride channel, voltage-sensitive 3
NCBI Official Symbol
Clcn3
NCBI Official Synonym Symbols
ClC-3
NCBI Protein Information
H(+)/Cl(-) exchange transporter 3; H(+)/Cl(-) exchange transporter 3; chloride channel 3; chloride channel protein 3; chloride transporter ClC-3; protein kinase C-regulated chloride channel
UniProt Protein Name
H(+)/Cl(-) exchange transporter 3
Protein Family
UniProt Gene Name
Clcn3
UniProt Synonym Gene Names
ClC-3
UniProt Entry Name
CLCN3_RAT
NCBI Description
mediates transepithelial chloride transport [RGD, Feb 2006]
Uniprot Description
CLCN3: Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory (By similarity)
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, ion channel; Vesicle; Transporter; Channel, chloride
Research Articles on Clcn3
Similar Products
Product Notes
The Clcn3 clcn3 (Catalog #AAA802814) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Clcn3 Antibody: ATTO 390 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Clcn3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). 1ug/mL (WB), 0.1-1ug/mL (IHC). Researchers should empirically determine the suitability of the Clcn3 clcn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clcn3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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