Western Blot (WB)
(Western Blot of MBS614021performed from 100ug of formic acid extracts of Alzheimer’s disease brain. Primary antibody MBS614021 (1:500 dilution) was incubated O/N at 4°C. Secondary rabbit HRP antibody was incubated 1hr at RT followed by ECL detection.)
Rabbit anti-Human, Mouse CLAC-P, NC2-2 Region Polyclonal Antibody
CLAC-P, NC2-2 Region (Collagen-like Alzheimer Amyloid Plaque Component)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CLAC-P; NC2-2 Region; Polyclonal Antibody; NC2-2 Region (Collagen-like Alzheimer Amyloid Plaque Component); Anti -CLAC-P; anti-CLAC-P; NC2-2 Region antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Reacts with residues 155-169 [KGEQGDQGPRMVFPK] of the NC2-2 region of human and mouse CLAC-P. Detects bands of 50, 70 and 100kD in SPs of AD brain, but not in diffuse plaques of non-demented aged individuals, diffuse deposits in sub-cortical structures and cerebellum in AD nor in cerebral amyloid angiopathy.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-CLAC-P, NC2-2 Region antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: Use at 1:500 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot of MBS614021performed from 100ug of formic acid extracts of Alzheimer’s disease brain. Primary antibody MBS614021 (1:500 dilution) was incubated O/N at 4°C. Secondary rabbit HRP antibody was incubated 1hr at RT followed by ECL detection.)
Western Blot (WB)
(Western Blot of MBS614021performed from 100ug of formic acid extracts of Alzheimer’s disease brain. Primary antibody MBS614021 (1:500 dilution) was incubated O/N at 4°C. Secondary rabbit HRP antibody was incubated 1hr at RT followed by ECL detection.)
Related Product Information for anti-CLAC-P, NC2-2 Region antibody
Antibodies against senile plaque (SP) amyloid obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with approximately 50/100kD polypeptides in Alzheimer's disease (AD) brains. The 9D2 antigen was identified as CLAC (collagen-like Alzheimer amyloid plaque component), and its precursor as CLAC-P/collagen type XXV. Both secreted and membrane-tethered forms of CLAC-P/collagen type XXV specifically bound to fibrillized Abeta, implicating these proteins in beta-amyloidogenesis and neuronal degeneration in AD.
Product Categories/Family for anti-CLAC-P, NC2-2 Region antibody
Similar Products
Product Notes
The CLAC-P, NC2-2 Region (Catalog #AAA614021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLAC-P, NC2-2 Region (Collagen-like Alzheimer Amyloid Plaque Component) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLAC-P, NC2-2 Region can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: Use at 1:500 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry. Researchers should empirically determine the suitability of the CLAC-P, NC2-2 Region for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLAC-P, NC2-2 Region, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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