Rabbit anti-Human; Mouse CISY Polyclonal Antibody
Anti-CISY (Citrate Synthase, Mitochondrial, CS)
Reactivity
Human; Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
CISY; Polyclonal Antibody; Anti-CISY (Citrate Synthase; Mitochondrial; CS); Citrate Synthase; CS; anti-CISY antibody
Host
Rabbit
Reactivity
Human; Mouse
Clonality
Polyclonal
Isotype
IgG1
Specificity
Recognizes mouse and human Citrate synthase.
Purity/Purification
Affinity Purified
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-CISY antibody
ELISA; Western Blot; Immunohistochemistry
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Recommended Dilutions: Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml
Immunohistochemistry: 1-20ug/ml
Recommended Dilutions: Western Blot: 1-10ug/ml (ECL)
ELISA: 0.5-1ug/ml
Immunohistochemistry: 1-20ug/ml
Immunogen
Synthetic peptide corresponding to 17aa from mouse CS (Accession # Q4TTW8; 466aa) and 20aa from human CS (Accession # O57390; 466aa).
Source Information
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-CISY antibody
The enzyme citrate synthase (E.C. 2.3.3.1 [previously 4.1.3.7]) is a pace-maker enzyme, as it controls the first committed step of the Krebs cycle, also called the citric acid cycle. Citrate synthase is localized within cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria. Citrate synthase catalyzes the condensation reaction of the two-carbon acetate residue from acetyl coenzyme A and a molecule of four-carbon oxaloacetate to form the sixcarbon citrate. Oxaloacetate will be regenerated after the completion of one round of the Krebs Cycle.
Human CS (chromosome 12p11-qter, 466aa precursor, mature chain 28-466aa) encodes a 466aa protein with 95% homology to its pig homolog. CS enzyme, a homodimer, is located at the Mitochondrion matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Human CS (chromosome 12p11-qter, 466aa precursor, mature chain 28-466aa) encodes a 466aa protein with 95% homology to its pig homolog. CS enzyme, a homodimer, is located at the Mitochondrion matrix. Citrate synthase is found in nearly all cells capable of oxidative metabolism.
Product Categories/Family for anti-CISY antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
citrate synthase
Similar Products
Product Notes
The CISY (Catalog #AAA627403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CISY (Citrate Synthase, Mitochondrial, CS) reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CISY can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot; Immunohistochemistry. Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Recommended Dilutions: Western Blot: 1-10ug/ml (ECL) ELISA: 0.5-1ug/ml Immunohistochemistry: 1-20ug/ml. Researchers should empirically determine the suitability of the CISY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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