Rabbit CIKS Polyclonal Antibody

CIKS, NT (Connection to IKK and SAPK, JNK, Act1, NF-kB Activator 1)

Cat. Num. AAA618045

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: CIKS; Polyclonal Antibody; NT (Connection to IKK and SAPK; JNK; Act1; NF-kB Activator 1); Anti -CIKS; anti-CIKS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Crossreacts with mouse and rat CIKS.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

• Applicable Applications for anti-CIKS antibody: Western Blot (WB)
• Application Notes: Positive control: human/mouse/rat kidney whole cell lysate. Western blot analysis of CIKS using antibody on 15 ugs of mouse kidney cell lysate. Observed size: ~60 kD; Dilution: Western blot: 1-2ug/ml
• Immunogen: Synthetic peptides to a portion of human CIKS.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CIKS using MBS618045 on 15 ugs of mouse kidney cell lysate.)

Related Product Information for anti-CIKS antibody

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (3) have isolated a cDNA coding for a protein named Act1 (NFkB activator 1). Act1/CIKS cDNA codes for a protein containing 574aa with a predicated molecular mass of 60kD. Act1/CIKS protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

Product Categories/Family for anti-CIKS antibody

Antibodies; Abs to Transcription Factors