Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Chymase 1 Polyclonal Antibody | anti-CMA1 antibody
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
Gene Names
Cma1; MCP3P; Mcpt5
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Chymase 1; Polyclonal Antibody; Polyclonal Antibody to Chymase 1; Mast Cell (CMA1); anti-CMA1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.91mg/ml (varies by lot)
Applicable Applications for anti-CMA1 antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2(Ug/mL;1:450-1800
Immunohistochemistry: 5-20(Ug/mL;1:45-180
Immunocytochemistry: 5-20(Ug/mL;1:45-180
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20(Ug/mL;1:45-180
Immunocytochemistry: 5-20(Ug/mL;1:45-180
Optimal working dilutions must be determined by end user.
Organism Species
Rattus norvegicus (Rat)
Source
Polyclonal antibody preparation
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Traits
Liquid
Immunogen
Recombinant CMA1 (Ile22~Asn247 (Accession # P50339)) expressed in E.coli
Cross Reactivity
Rat
Conjugated Antibody
The APC-Cy7 conjugated antibody version of this item is also available as catalog #MBS2078264
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
chymase
NCBI Official Synonym Full Names
chymase 1
NCBI Official Symbol
Cma1
NCBI Official Synonym Symbols
MCP3P; Mcpt5
NCBI Protein Information
chymase
UniProt Protein Name
Chymase
Protein Family
UniProt Gene Name
Cma1
UniProt Synonym Gene Names
Mcpt3; rMCP-3; rMCP-5; rMCP-III
NCBI Description
connective tissue mast cell protein with proteolytic activity; may facilitate apoptosis [RGD, Feb 2006]
Uniprot Description
CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: EC 3.4.21.39; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15p13
Cellular Component: extracellular matrix; extracellular region; intracellular; secretory granule
Molecular Function: endopeptidase activity; peptidase activity; peptide binding; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: interleukin-1 beta biosynthetic process; midbrain development; peptide metabolic process; positive regulation of angiogenesis; proteolysis
Research Articles on CMA1
Similar Products
Product Notes
The CMA1 cma1 (Catalog #AAA2027557) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Chymase 1, Mast Cell (CMA1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Chymase 1 can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2(Ug/mL;1:450-1800Immunohistochemistry: 5-20(Ug/mL;1:45-180
Immunocytochemistry: 5-20(Ug/mL;1:45-180
Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CMA1 cma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chymase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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