Typical Testing Data/Standard Curve (for reference only)
Rat Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2) ELISA Kit | ACOT2 elisa kit
Rat Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AchRAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Acetylcholine Receptor antigen and an AchRAb-HRP conjugate. The assay sample and buffer are incubated together with AchRAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AchRAb concentration since AchRAb from samples and AchRAb-HRP conjugate compete for the Acetylcholine Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by AchRAb from the sample, fewer sites are left to bind AchRAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AchRAb concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
ACOT2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs. Belongs to the C/M/P thioester hydrolase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - unsaturated fatty acid biosynthesis; Hydrolase; EC 3.1.2.2; Mitochondrial
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: mitochondrion
Molecular Function: acyl-CoA hydrolase activity; palmitoyl-CoA hydrolase activity; receptor binding
Biological Process: acyl-CoA metabolic process; very-long-chain fatty acid metabolic process; long-chain fatty acid metabolic process
Research Articles on ACOT2
Similar Products
Product Notes
The Rat ACOT2 acot2 (Catalog #AAA7202446) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7202446 ELISA Kit recognizes Rat ACOT2. It is sometimes possible for the material contained within the vial of "Acyl-coenzyme A thioesterase 2, mitochondrial (ACOT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
