Rabbit anti-Rat Galectin-1 Polyclonal Antibody | anti-LGALS1 antibody

Anti-Galectin-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8112862

• Reactivity: Rat
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Galectin-1; Polyclonal Antibody; Anti-Galectin-1 Antibody; Rabbit Polyclonal; Galectin-1/LGALS1 Antibody; Rabbit PAb; Antigen Affinity Purified; lectin; galactoside-binding; soluble; 1; anti-LGALS1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat Galectin-1/LGALS1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LGALS1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Rat Galectin-1/LGALS1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat Galectin-1/LGALS1 (rR Galectin-1/LGALS1). Galectin-1/LGALS1 specific IgG was purified by Rat Galectin-1/LGALS1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(rat Galectin1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-rat Galectin1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-rat Galectin1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 15 kDaObserved band size: 15 kDa)

Western Blot (WB)

(Anti-Galectin1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:15 kDaObserved band size:14 kDa)

Related Product Information for anti-LGALS1 antibody

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.

References

Gaudet AD, et al. (2005) Expression and functions of galectin-1 in sensory and motoneurons. Curr Drug Targets. 6(4): 419-25.Kadoya T, et al. (2006) Structural and functional studies of galectin-1: a novel axonal regeneration-promoting activity for oxidized galectin-1. Curr Drug Targets. 6(4): 375-83.Camby I, et al. (2006) Galectin-1: a small protein with major functions. Glycobiology. 16(11): 137R-157R.Salatino M, et al. (2008) Galectin-1 as a potential therapeutic target in autoimmune disorders and cancer. Expert Opin Biol Ther. 8(1): 45-57.

NCBI and Uniprot Product Information

• NCBI GI #: 9845261
• NCBI GeneID: 56646
• NCBI Accession #: NP_063969.1
• NCBI GenBank Nucleotide #: NM_019904.1
• UniProt Accession #: P11762
• Molecular Weight: 14,857 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: Lgals1
• NCBI Protein Information: galectin-1
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: Lgals1
• UniProt Synonym Gene Names: Gal-1
• UniProt Entry Name: LEG1_RAT

NCBI Description

lectin that binds beta-galactoside [RGD, Feb 2006]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Extracellular matrix; Secreted; Cell adhesion

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular space; intracellular; nucleus; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; glycoprotein binding; lactose binding; laminin binding; protein homodimerization activity; signal transducer activity

Biological Process: apoptosis; maintenance of protein localization; multicellular organismal response to stress; myoblast differentiation; plasma cell differentiation; positive regulation of erythrocyte aggregation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of virion penetration into host cell; response to axon injury; response to drug; signal transduction; T cell costimulation