Rabbit anti-Human CHST8 Polyclonal Antibody | anti-CHST8 antibody

CHST8, ID (CHST8, Carbohydrate sulfotransferase 8, GalNAc-4-O-sulfotransferase 1, N-acetylgalactosamine-4-O-sulfotransferase 1) (Azide free) (HRP)

Cat. Num. AAA6285990

• Gene Names: CHST8; PSS3; GalNAc4ST; GALNAC4ST1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHST8; Polyclonal Antibody; ID (CHST8; Carbohydrate sulfotransferase 8; GalNAc-4-O-sulfotransferase 1; N-acetylgalactosamine-4-O-sulfotransferase 1) (Azide free) (HRP); N-acetylgalactosamine-4-O-sulfotransferase 1; anti-CHST8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 424

• Applicable Applications for anti-CHST8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHST8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human CHST8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CHST8 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CHST8 antibody detected the CHST8 protein (arrow).)

Related Product Information for anti-CHST8 antibody

Sulfate groups in carbohydrates confer highly specific functions on glycoproteins, glycolipids, and proteoglycans and are critical for cell-cell interaction, signal transduction, and embryonic development. Sulfotransferases, such as CHST8, carry out sulfation of carbohydrates (Hiraoka et al., 2001 [PubMed 11445554]).

Product Categories/Family for anti-CHST8 antibody

Antibodies; Enzymes; Sulfotransferase

References

Melen, E., et al. J. Allergy Clin. Immunol. 126(3):631-637(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Thorleifsson, G., et al. Nat. Genet. 41(1):18-24(2009). Boregowda, R.K., et al. Glycobiology 15(12):1349-1358(2005). Barret, A., et al. J. Biol. Chem. 280(11):10516-10523(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 20127609
• NCBI GeneID: 64377
• NCBI Accession #: NP_071912.2; NP_001121367.1; NP_001121368.1
• NCBI GenBank Nucleotide #: NP_071912.2
• UniProt Accession #: Q9H2A9
• NCBI Official Full Name: carbohydrate sulfotransferase 8
• NCBI Official Synonym Full Names: carbohydrate sulfotransferase 8
• NCBI Official Symbol: CHST8
• NCBI Official Synonym Symbols: PSS3; GalNAc4ST; GALNAC4ST1
• NCBI Protein Information: carbohydrate sulfotransferase 8
• UniProt Protein Name: Carbohydrate sulfotransferase 8
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST8
• UniProt Synonym Gene Names: GalNAc-4-ST1; GalNAc4ST-1
• UniProt Entry Name: CHST8_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sulfotransferase 2 family. It is predominantly expressed in the pituitary gland, and is localized to the golgi membrane. This protein catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. It is responsible for sulfation of GalNAc on luteinizing hormone (LH), which is required for production of the sex hormones. Mice lacking this enzyme, exhibit increased levels of circulating LH, and precocious sexual maturation of both male and female mice. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CHST8: Catalyzes the transfer of sulfate to position 4 of non- reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin. CHST8 mutations may be a cause of generalized non- inflammatory peeling skin syndrome type A (PubMed:22289416). Peeling skin syndrome (PSS) is a genodermatosis characterized by continuous shedding of the outer layers of the epidermis. Two main PSS subtypes have been suggested. Patients with non-inflammatory PSS (type A) manifest white scaling, with painless and easy removal of the skin, irritation when in contact with water, dust and sand, and no history of erythema, pruritis or atopy. Inflammatory PSS (type B) is associated with generalized erythema, pruritus and atopy. It is an ichthyosiform erythroderma characterized by lifelong patchy peeling of the entire skin with onset at birth or shortly after. Several patients have been reported with high IgE levels. Belongs to the sulfotransferase 2 family.

Protein type: Transferase; EC 2.8.2.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: N-acetylgalactosamine 4-O-sulfotransferase activity

Biological Process: sulfur metabolic process; central nervous system development; proteoglycan biosynthetic process; hormone biosynthetic process; carbohydrate biosynthetic process

Disease: Peeling Skin Syndrome 3