Rabbit CHST15 Polyclonal Antibody | anti-CHST15 antibody

CHST15 Antibody

Cat. Num. AAA9611649

• Gene Names: CHST15; BRAG; GALNAC4S-6ST
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CHST15; Polyclonal Antibody; CHST15 Antibody; B cell RAG associated gene protein; B cell RAG associated protein; B-cell RAG-associated gene protein; BRAG; Carbohydrate sulfotransferase 15; Chst15; CHSTF_HUMAN; GALNAC4S 6ST; GalNAc4S-6ST; hBRAG; KIAA0598; N acetylgalactosamine 4 sulfate 6 O sulfotransferase; N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase; ST4S6; anti-CHST15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHST15 Antibody detects endogenous levels of CHST15.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 561

• Applicable Applications for anti-CHST15 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human CHST15.
• Tissue Specificity: Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Golgi Apparatus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from ?Jurkat cells, using CHST15 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-CHST15 antibody

Description: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.

Post Translational Modifications: Glycosylated.

Subunit Structure: Homodimer; disulfide-linked (Potential). The relevance of homodimerization is however unsure. May interact with phosphorylated proteins in resting B-cells, including HCK.

Similarity: Belongs to the sulfotransferase 1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 398303854
• NCBI GeneID: 51363
• NCBI Accession #: NP_001257693
• NCBI GenBank Nucleotide #: NP_001257693.1
• UniProt Accession #: Q7LFX5
• Molecular Weight: Observed: 69 kDa; Predicted: 65 kDa
• NCBI Official Full Name: carbohydrate sulfotransferase 15 isoform 1
• NCBI Official Synonym Full Names: carbohydrate sulfotransferase 15
• NCBI Official Symbol: CHST15
• NCBI Official Synonym Symbols: BRAG; GALNAC4S-6ST
• NCBI Protein Information: carbohydrate sulfotransferase 15
• UniProt Protein Name: Carbohydrate sulfotransferase 15
• Protein Family: Carbohydrate sulfotransferase
• UniProt Gene Name: CHST15
• UniProt Synonym Gene Names: BRAG; GALNAC4S6ST; KIAA0598; hBRAG; GalNAc4S-6ST
• UniProt Entry Name: CHSTF_HUMAN

NCBI Description

Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]