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Western Blot (WB) (CHRNB4 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRNB4 antibody detected the CHRNB4 protein (arrow).)

Rabbit anti-Human CHRNB4 Polyclonal Antibody | anti-CHRNB4 antibody

CHRNB4, CT (CHRNB4, Neuronal acetylcholine receptor subunit beta-4) (PE)

Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CHRNB4; Polyclonal Antibody; CT (CHRNB4; Neuronal acetylcholine receptor subunit beta-4) (PE); Neuronal acetylcholine receptor subunit beta-4; anti-CHRNB4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CHRNB4 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
CHRNB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 422-450 amino acids from the C-terminal region of human CHRNB4.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CHRNB4 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRNB4 antibody detected the CHRNB4 protein (arrow).)

Western Blot (WB) (CHRNB4 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHRNB4 antibody detected the CHRNB4 protein (arrow).)
Related Product Information for anti-CHRNB4 antibody
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Product Categories/Family for anti-CHRNB4 antibody
References
Zhang, H., et al. Neuropsychopharmacology 35(11):2211-2224(2010). Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010). Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010). Amos, C.I., et al. J. Natl. Cancer Inst. 102(15):1199-1205(2010). Li, M.D., et al. PLoS ONE 5 (8), E12183 (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,862 Da
NCBI Official Full Name
neuronal acetylcholine receptor subunit beta-4 isoform 1
NCBI Official Synonym Full Names
cholinergic receptor, nicotinic, beta 4 (neuronal)
NCBI Official Symbol
CHRNB4
NCBI Protein Information
neuronal acetylcholine receptor subunit beta-4; neuronal nicotinic receptor beta 4 subunit; cholinergic receptor, nicotinic, beta polypeptide 4; acetylcholine receptor, nicotinic, beta 4 (neuronal)
UniProt Protein Name
Neuronal acetylcholine receptor subunit beta-4
UniProt Gene Name
CHRNB4
UniProt Entry Name
ACHB4_HUMAN

Uniprot Description

nAChRB4: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 4/CHRNB4 sub-subfamily.

Protein type: Receptor, misc.; Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral; Channel, cation

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to plasma membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; ligand-gated ion channel activity

Biological Process: regulation of smooth muscle contraction; synaptic transmission; regulation of action potential; smooth muscle contraction; regulation of neurotransmitter secretion; behavioral response to nicotine; locomotory behavior; ion transport; synaptic transmission involved in micturition; signal transduction; cation transport; synaptic transmission, cholinergic

Research Articles on CHRNB4

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Product Notes

The CHRNB4 chrnb4 (Catalog #AAA6285886) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHRNB4, CT (CHRNB4, Neuronal acetylcholine receptor subunit beta-4) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRNB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CHRNB4 chrnb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRNB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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