Rabbit anti-Human CHRNA3 Polyclonal Antibody | anti-CHRNA3 antibody

CHRNA3, NT (CHRNA3, NACHRA3, Neuronal acetylcholine receptor subunit alpha-3) (Azide free) (HRP)

Cat. Num. AAA6285847

• Gene Names: CHRNA3; LNCR2; PAOD2; NACHRA3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHRNA3; Polyclonal Antibody; NT (CHRNA3; NACHRA3; Neuronal acetylcholine receptor subunit alpha-3) (Azide free) (HRP); Neuronal acetylcholine receptor subunit alpha-3; anti-CHRNA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CHRNA3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-60 amino acids from the N-terminal region of human CHRNA3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CHRNA3 Antibody (N-term) in CEM cell line lysates (35ug/lane).CHRNA3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(CHRNA3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRNA3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CHRNA3 antibody

CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Product Categories/Family for anti-CHRNA3 antibody

Antibodies; Receptors

References

Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010). Girard, N., et al. Clin. Cancer Res. 16(2):755-763(2010). Grando, S.A., et al. J. Invest. Dermatol. 105(6):774-781(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 254763435
• NCBI GeneID: 1136
• UniProt Accession #: P32297; Q15823; Q4KMN8; Q86U77; Q96RH3; Q99553; Q9BQ93; Q9BRR4
• Molecular Weight: 505
• NCBI Official Full Name: Neuronal acetylcholine receptor subunit alpha-3
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 3 (neuronal)
• NCBI Official Symbol: CHRNA3
• NCBI Official Synonym Symbols: LNCR2; PAOD2; NACHRA3
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-3; cholinergic receptor, nicotinic, alpha polypeptide 3; neuronal nicotinic acetylcholine receptor, alpha3 subunit
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA3
• UniProt Synonym Gene Names: NACHRA3
• UniProt Entry Name: ACHA3_HUMAN

NCBI Description

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Uniprot Description

nAChRA3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 3/CHRNA3 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, integral; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; cell soma; dendrite; postsynaptic density; plasma membrane; integral to membrane; cell junction

Molecular Function: acetylcholine receptor activity; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity; ligand-gated ion channel activity

Biological Process: regulation of smooth muscle contraction; nervous system development; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of dendrite morphogenesis; regulation of acetylcholine secretion; behavioral response to nicotine; locomotory behavior; signal transduction; synaptic transmission, cholinergic; synaptic transmission; regulation of membrane potential; ion transport; synaptic transmission involved in micturition; regulation of excitatory postsynaptic membrane potential

Disease: Smoking As A Quantitative Trait Locus 3