Rabbit anti-Human CHRNA10 Polyclonal Antibody | anti-CHRNA10 antibody

CHRNA10, ID (CHRNA10, NACHRA10, Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10) (APC)

Cat. Num. AAA6285822

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHRNA10; Polyclonal Antibody; ID (CHRNA10; NACHRA10; Neuronal acetylcholine receptor subunit alpha-10; Nicotinic acetylcholine receptor subunit alpha-10) (APC); Nicotinic acetylcholine receptor subunit alpha-10; anti-CHRNA10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CHRNA10 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CHRNA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human CHRNA10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(CHRNA10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CHRNA10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(CHRNA10 Antibody (Center) western blot analysis in NCI-H292,MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the CHRNA10 antibody detected the CHRNA10 protein (arrow).)

Related Product Information for anti-CHRNA10 antibody

CHRNA10 is an ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.

Product Categories/Family for anti-CHRNA10 antibody

Antibodies; Receptors; Cholinergic

References

Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Rigbi, A., et al. Pharmacogenomics J. (2010). In press: Need, A.C., et al. Eur. J. Hum. Genet. 17(7):946-957(2009). Saccone, N.L., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (4), 453-466 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 11138123
• NCBI GeneID: 57053
• NCBI Accession #: NP_065135.2
• NCBI GenBank Nucleotide #: NM_020402.2
• UniProt Accession #: Q9GZZ6
• Molecular Weight: 49,705 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-10
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 10 (neuronal)
• NCBI Official Symbol: CHRNA10
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-10; NACHR alpha-10; nicotinic acetylcholine receptor subunit alpha-10; cholinergic receptor, nicotinic, alpha polypeptide 10; acetylcholine receptor, nicotinic, alpha 10 (neuronal)
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-10
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA10
• UniProt Synonym Gene Names: NACHRA10; NACHR alpha-10
• UniProt Entry Name: ACH10_HUMAN

Uniprot Description

nAChRA10: Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 10/CHRNA10 sub-subfamily.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; membrane; axon; perikaryon; cell junction

Molecular Function: calcium channel activity; nicotinic acetylcholine-activated cation-selective channel activity; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; inner ear morphogenesis; detection of mechanical stimulus involved in sensory perception of sound; regulation of cell proliferation; synaptic transmission, cholinergic