Rabbit CHRNA10 Polyclonal Antibody | anti-CHRNA10 antibody

Anti-CHRNA10 Antibody (aa394-443) IHC-plus

Cat. Num. AAA2400442

• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity purified
• Synonyms: CHRNA10; Polyclonal Antibody; Anti-CHRNA10 Antibody (aa394-443) IHC-plus; Rabbit Polyclonal (IgG) to Human CHRNA10; Human CHRNA10; AChR-alpha10; NACHRA10; NACHR alpha-10; anti-CHRNA10 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CHRNA10 Antibody detects endogenous levels of total CHRNA10 protein.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-CHRNA10 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:20000); IHC-P (10 ug/ml); WB (1:500 - 1:1000)
• Immunogen: CHRNA10 antibody was raised against synthetic peptide from human CHRNA10 (aa394-443).
• Immunogen Description: Synthetic peptide from human CHRNA10 (aa394-443).
• Antigen Modification: aa394-443
• Target Species: Human
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-CHRNA10 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HeLa/293/COLO/A549 cells, using CHRNA10 Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-CHRNA10 antibody

CHRNA10 Antibody, AChR-alpha10 Antibody, NACHRA10 Antibody, NACHR alpha-10 Antibody Description: Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 11138123
• NCBI GeneID: 57053
• NCBI Accession #: NP_065135.2
• NCBI GenBank Nucleotide #: NM_020402.3
• UniProt Accession #: Q9GZZ6
• Molecular Weight: 49,705 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-10 isoform a
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 10 subunit
• NCBI Official Symbol: CHRNA10
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-10
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-10
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA10
• UniProt Synonym Gene Names: NACHRA10; NACHR alpha-10
• UniProt Entry Name: ACH10_HUMAN

Uniprot Description

nAChRA10: Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 10/CHRNA10 sub-subfamily.

Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: axon; cell junction; cytosol; membrane; nicotinic acetylcholine-gated receptor-channel complex; perikaryon; postsynaptic membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; calcium channel activity; nicotinic acetylcholine-activated cation-selective channel activity; receptor binding

Biological Process: detection of mechanical stimulus involved in sensory perception of sound; elevation of cytosolic calcium ion concentration; inner ear morphogenesis; regulation of cell proliferation; synaptic transmission, cholinergic