Rabbit CHRDL2 Polyclonal Antibody | anti-CHRDL2 antibody

CHRDL2 Polyclonal Antibody

Cat. Num. AAA2523242

• Gene Names: CHRDL2; BNF1; CHL2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: CHRDL2; Polyclonal Antibody; CHRDL2 Polyclonal Antibody; BNF1; CHL2; FKSG37; anti-CHRDL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.2mg/mL (varies by lot)
• Sequence Length: 386

• Applicable Applications for anti-CHRDL2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:25-1:100
• Immunogen: Synthetic peptide of human CHRDL2
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Human fetal brain tissue using CHRDL2 Polyclonal Antibody at dilution of 1:600)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer using CHRDL2 Polyclonal Antibody at dilution of 1:20)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colon cancer using CHRDL2 Polyclonal Antibody at dilution of 1:20)

Related Product Information for anti-CHRDL2 antibody

CHRDL2 (chordin-related protein 2), also known as BNF1 (breast tumor novel factor 1) or CHL2 (chordin-like 2), is a 429 amino acid protein that belongs to the chordin family of proteins. CHRDL2 contains three VWFC (von Willebrand Factor type C) domains and is predominantly expressed in uterus and moderately expressed in prostate, liver, ovary, heart and testis. Due to alternative splicing events, CHRDL2 exists as five isoforms, namely isoform I, isoform II, isoform VII, isoform VIII and isoform IX. Two of these isoforms are secreted, while the other three localize to the cytoplasm. Functioning as a BMP-binding inhibitor, CHRDL2 directly interacts with BMPs and blocks their binding to BMP receptors, thereby inhibiting BMP activity. In breast, lung and colon tumors, CHRDL2 expression is upregulated, suggesting a possible role in tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 749502305
• NCBI GeneID: 25884
• NCBI Accession #: NP_001291346.1
• NCBI GenBank Nucleotide #: NM_001304417.1
• Molecular Weight: 9,846 Da
• NCBI Official Full Name: chordin-like protein 2 isoform 7
• NCBI Official Synonym Full Names: chordin-like 2
• NCBI Official Symbol: CHRDL2
• NCBI Official Synonym Symbols: BNF1; CHL2
• NCBI Protein Information: chordin-like protein 2; breast tumor novel factor 1; chordin-related protein 2
• UniProt Protein Name: Chordin-like protein 2
• Protein Family: Chordin-like protein
• UniProt Gene Name: CHRDL2
• UniProt Synonym Gene Names: BNF1; CHL2; BNF-1
• UniProt Entry Name: CRDL2_HUMAN

NCBI Description

This gene encodes a member of the chordin family of proteins. Chordin family members are secreted proteins that share a cysteine-rich pro-collagen repeat domain and associate with members of the transforming growth factor beta superfamily. In vitro assays demonstrate a direct interaction between the encoded protein and human activin A. This gene is expressed in many tissues including osteoblasts, where it is differentially expressed during differentiation. In addition, its expression is upregulated in human osteoarthritic joint cartilage, suggesting a role in adult cartilage regeneration. [provided by RefSeq, Jan 2015]

Uniprot Description

CHRDL2: May inhibit BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. Implicated in tumor angiogenesis. May play a role during myoblast and osteoblast differentiation, and maturation. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q14

Cellular Component: extracellular space; cytoplasm

Biological Process: ossification; cartilage development; cell differentiation; negative regulation of BMP signaling pathway