Rabbit anti-Human ALDH6A1 Polyclonal Antibody | anti-Aldh6a1 antibody

ALDH6A1, NT (ALDH6A1, MMSDH, Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, Aldehyde dehydrogenase family 6 member A1)

Cat. Num. AAA6003939

• Gene Names: Aldh6a1; Mmsdh; AI314632; 1110038I05Rik
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ALDH6A1; Polyclonal Antibody; NT (ALDH6A1; MMSDH; Methylmalonate-semialdehyde dehydrogenase [acylating]; mitochondrial; Aldehyde dehydrogenase family 6 member A1); Anti -ALDH6A1; anti-Aldh6a1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Aldh6a1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: ALDH6A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37~67 amino acids from the N-terminal region of human ALDH6A1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALDH6A1 Antibody (N-term) in T47D cell line lysates (35ug/lane). ALDH6A1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with *ALDH6A1 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Aldh6a1 antibody

ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. This protein is a mitochondrial methylmalonate semialdehyde dehydrogenase, and catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

Product Categories/Family for anti-Aldh6a1 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21410418
• NCBI GeneID: 104776
• UniProt Accession #: Q9EQ20; Q3TDA2; Q8CIB4
• Molecular Weight: 57,916 Da
• NCBI Official Full Name: Aldh6a1 protein
• NCBI Official Synonym Full Names: aldehyde dehydrogenase family 6, subfamily A1
• NCBI Official Symbol: Aldh6a1
• NCBI Official Synonym Symbols: Mmsdh; AI314632; 1110038I05Rik
• NCBI Protein Information: methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; aldehyde dehydrogenase family 6 member A1
• UniProt Protein Name: Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
• UniProt Gene Name: Aldh6a1
• UniProt Synonym Gene Names: MMSDH
• UniProt Entry Name: MMSA_MOUSE

NCBI Description

This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded enzyme is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This enzyme catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Mutations in the human gene result in Methylmalonate Semialdehyde Dehydrogenase Deficiency, characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

ALDH6A1: Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA. Defects in ALDH6A1 are the cause of methylmalonate semialdehyde dehydrogenase deficiency (MMSDHD). This is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Belongs to the aldehyde dehydrogenase family.

Protein type: EC 1.2.1.18; Carbohydrate Metabolism - inositol phosphate; Mitochondrial; Carbohydrate Metabolism - propanoate; EC 1.2.1.27; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: aldehyde dehydrogenase (NAD) activity; thiolester hydrolase activity; methylmalonate-semialdehyde dehydrogenase (acylating) activity; malonate-semialdehyde dehydrogenase (acetylating) activity; oxidoreductase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Biological Process: valine catabolic process; metabolic process; thymine metabolic process; brown fat cell differentiation; valine metabolic process; thymine catabolic process; beta-alanine catabolic process