Western Blot (WB)
(Western blot analysis of CHPF Antibody (Center) in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane). CHPF (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse CHPF Polyclonal Antibody | anti-CHPF antibody
CHPF, ID (CHPF, CSS2, Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3
Gene Names
CHPF; CSS2; CHSY2
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CHPF; Polyclonal Antibody; ID (CHPF; CSS2; Chondroitin sulfate synthase 2; Chondroitin glucuronyltransferase 2; Chondroitin-polymerizing factor; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-proteoglycan 3; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II; N-acetylgalactosaminyltransferase 2; anti-CHPF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-CHPF antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
CHPF antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human CHPF.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CHPF Antibody (Center) in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane). CHPF (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of CHPF Antibody (Center) in MDA-MB435, MCF-7, HepG2, A375 cell line and mouse testis tissue lysates (35ug/lane). CHPF (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CHPF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with CHPF Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(CHPF Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(CHPF Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-CHPF antibody
CHPF is a protein that has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.
Product Categories/Family for anti-CHPF antibody
References
Matsuoka, S., et.al., Science 316 (5828), 1160-1166 (2007). Colland, F., et.al., Genome Res. 14 (7), 1324-1332 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85,467 Da
NCBI Official Full Name
chondroitin sulfate synthase 2 isoform 2
NCBI Official Synonym Full Names
chondroitin polymerizing factor
NCBI Official Symbol
CHPF
NCBI Official Synonym Symbols
CSS2; CHSY2
NCBI Protein Information
chondroitin sulfate synthase 2; chondroitin-polymerizing factor; N-acetylgalactosaminyltransferase 2; chondroitin glucuronyltransferase 2; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II; glucuronosyl-N-acetylgalactosaminyl-proteogly
UniProt Protein Name
Chondroitin sulfate synthase 2
Protein Family
UniProt Gene Name
CHPF
UniProt Synonym Gene Names
CSS2; ChPF
UniProt Entry Name
CHSS2_HUMAN
Uniprot Description
CHPF: a type II transmembrane protein. May act as a specific activating factor for chondroitin synthase in chondroitin biosynthesis and polymerization.
Protein type: Transferase; EC 2.4.1.226; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; EC 2.4.1.175
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: Golgi membrane; mitochondrial matrix; integral to membrane; cytosol
Molecular Function: protein binding; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; metal ion binding; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity
Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on CHPF
Similar Products
Product Notes
The CHPF chpf (Catalog #AAA6285768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHPF, ID (CHPF, CSS2, Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHPF can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CHPF chpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHPF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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