Western Blot (WB)
(Western blot analysis of extracts from LOVO cells and K562 cells, using CHSS2 antibody.)
Rabbit CHSS2 Polyclonal Antibody | anti-CHSS2 antibody
CHSS2 Antibody
Gene Names
CHPF; CSS2; CHSY2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CHSS2; Polyclonal Antibody; CHSS2 Antibody; DKFZP434E0423; EC 2.4.1.175; EC 2.4.1.226; anti-CHSS2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total CHSS2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
613
Applicable Applications for anti-CHSS2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human CHSS2.
Target Name
CHSS2
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells and K562 cells, using CHSS2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells and K562 cells, using CHSS2 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using CHSS2 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue using CHSS2 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells, using CHSS2 antibody.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells, using CHSS2 antibody.)
Related Product Information for anti-CHSS2 antibody
Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Isoform 2 may facilitate PARK2 transport into the mitochondria. In collaboration with PARK2, isoform 2 may enhance cell viability and protect cells from oxidative stress.Kitagawa H., J. Biol. Chem. 278:23666-23671(2003).Yada T., J. Biol. Chem. 278:30235-30247(2003).Clark H.F., Genome Res. 13:2265-2270(2003)
Product Categories/Family for anti-CHSS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,341 Da
NCBI Official Full Name
chondroitin sulfate synthase 2 isoform 2
NCBI Official Synonym Full Names
chondroitin polymerizing factor
NCBI Official Symbol
CHPF
NCBI Official Synonym Symbols
CSS2; CHSY2
NCBI Protein Information
chondroitin sulfate synthase 2
UniProt Protein Name
Chondroitin sulfate synthase 2
UniProt Gene Name
CHPF
UniProt Synonym Gene Names
CSS2; ChPF
UniProt Entry Name
CHSS2_HUMAN
Uniprot Description
CHPF: a type II transmembrane protein. May act as a specific activating factor for chondroitin synthase in chondroitin biosynthesis and polymerization.
Protein type: Membrane protein, integral; EC 2.4.1.226; EC 2.4.1.175; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: Golgi membrane; mitochondrial matrix; integral to membrane; cytosol
Molecular Function: protein binding; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; metal ion binding; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity
Biological Process: chondroitin sulfate metabolic process; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis
Research Articles on CHSS2
Similar Products
Product Notes
The CHSS2 chpf (Catalog #AAA9411469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHSS2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHSS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:500. Researchers should empirically determine the suitability of the CHSS2 chpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHSS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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