Rabbit CHP1 Polyclonal Antibody | anti-CHP1 antibody

CHP1 Antibody

Cat. Num. AAA7051104

• Gene Names: CHP1; CHP; p22; p24; Sid470p; SLC9A1BP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: CHP1; Polyclonal Antibody; CHP1 Antibody; Calcineurin B homologous protein 1; Calcineurin B-like protein; Calcium-binding protein CHP; Calcium-binding protein p22; EF-hand calcium-binding domain-containing protein p22; CHP; anti-CHP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 195

• Applicable Applications for anti-CHP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1: 500-1: 5000; IHC: 1: 20-1: 200; IF: 1: 50-1: 200
• Immunogen: Recombinant Human Calcineurin B homologous protein 1 protein (2-195AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160240
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat kidney tissue,Mouse brain tissueAll lanes: CHP1 antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 23 KDaObserved band size: 23 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7051104 diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7051104 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-CHP1 antibody

Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.

NCBI and Uniprot Product Information

• NCBI GI #: 6005731
• NCBI GeneID: 11261
• NCBI Accession #: NP_009167.1
• NCBI GenBank Nucleotide #: NM_007236.4
• UniProt Accession #: Q99653; Q6FHZ9; B2R6H9
• Molecular Weight: 22,456 Da
• NCBI Official Full Name: calcineurin B homologous protein 1
• NCBI Official Synonym Full Names: calcineurin like EF-hand protein 1
• NCBI Official Symbol: CHP1
• NCBI Official Synonym Symbols: CHP; p22; p24; Sid470p; SLC9A1BP
• NCBI Protein Information: calcineurin B homologous protein 1
• UniProt Protein Name: Calcineurin B homologous protein 1
• Protein Family: Calcineurin B homologous protein
• UniProt Gene Name: CHP1
• UniProt Synonym Gene Names: CHP
• UniProt Entry Name: CHP1_HUMAN

NCBI Description

This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq, Jul 2008]

Uniprot Description

CHP: Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate- phosphorylations in a calcium-dependent manner. Belongs to the calcineurin regulatory subunit family. CHP subfamily.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 15q13.3

Cellular Component: cytoplasm; endoplasmic reticulum; focal adhesion; Golgi membrane; microtubule cytoskeleton; nucleus; plasma membrane; transport vesicle

Molecular Function: calcium ion binding; calcium-dependent protein binding; kinase binding; microtubule binding; potassium channel regulator activity; protein binding; transporter activity

Biological Process: cytoplasmic microtubule organization and biogenesis; hyaluronan catabolic process; inhibition of NF-kappaB transcription factor; membrane docking; microtubule bundle formation; negative regulation of protein amino acid autophosphorylation; negative regulation of protein amino acid phosphorylation; negative regulation of protein import into nucleus; negative regulation of protein kinase activity; negative regulation of protein ubiquitination; positive regulation of protein amino acid glycosylation; positive regulation of protein transport; positive regulation of sodium:hydrogen antiporter activity; potassium ion transport; protein export from nucleus; protein stabilization; regulation of intracellular pH; small GTPase mediated signal transduction