Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein phosphatase Slingshot homolog 1 (SSH1) Standard Curve.)
Human Protein Phosphatase Slingshot Homolog 1 (SSH1) ELISA Kit | SSH1 elisa kit
Human Protein Phosphatase Slingshot Homolog 1 (SSH1) ELISA Kit
Gene Names
SSH1; SSH1L
Reactivity
Human
Synonyms
Protein Phosphatase Slingshot Homolog 1 (SSH1); Human Protein Phosphatase Slingshot Homolog 1 (SSH1) ELISA Kit; SSH1; FLJ21928; FLJ38102; KIAA1298; SSH1L; slingshot 1; SSH1 elisa kit
Reactivity
Human
Specificity
Human Protein phosphatase Slingshot homolog 1 (SSH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSH1. No significant cross-reactivity or interference between Human SSH1 and analogues was observed.
Sequence Length
692
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein phosphatase Slingshot homolog 1 (SSH1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein phosphatase Slingshot homolog 1 (SSH1) Standard Curve.)
Related Product Information for SSH1 elisa kit
Description: This Human Protein phosphatase Slingshot homolog 1 (SSH1) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH1 in samples. An antibody specific for SSH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SSH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo. Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L (1,049 amino acids), SSH1S (692 amino acids), and SSH1B (148 amino acids), and SSH2 encodes 2 isoforms, SSH2 (449 amino acids) and SSH2B (195 amino acids). The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs and are unique to the SSH family.
Background: The ADF (actin-depolymerizing factor)/cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo. Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L (1,049 amino acids), SSH1S (692 amino acids), and SSH1B (148 amino acids), and SSH2 encodes 2 isoforms, SSH2 (449 amino acids) and SSH2B (195 amino acids). The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs and are unique to the SSH family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein phosphatase Slingshot homolog 1 isoform 2
NCBI Official Synonym Full Names
slingshot protein phosphatase 1
NCBI Official Symbol
SSH1
NCBI Official Synonym Symbols
SSH1L
NCBI Protein Information
protein phosphatase Slingshot homolog 1
UniProt Protein Name
Protein phosphatase Slingshot homolog 1
Protein Family
UniProt Gene Name
SSH1
UniProt Synonym Gene Names
KIAA1298; SSH1L; SSH-1L; hSSH-1L
NCBI Description
The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
Research Articles on SSH1
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Product Notes
The Human SSH1 ssh1 (Catalog #AAA9714032) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714032 ELISA Kit recognizes Human SSH1. It is sometimes possible for the material contained within the vial of "Protein Phosphatase Slingshot Homolog 1 (SSH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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