Rabbit anti-Human CHFR Polyclonal Antibody | anti-CHFR antibody

CHFR, CT (CHFR, RNF196, E3 ubiquitin-protein ligase CHFR, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196) (APC)

Cat. Num. AAA6285591

• Gene Names: CHFR; RNF116; RNF196
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHFR; Polyclonal Antibody; CT (CHFR; RNF196; E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196) (APC); RING finger protein 196; anti-CHFR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 652

• Applicable Applications for anti-CHFR antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 475-504 amino acids from the C-terminal region of human CHFR.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CHFR Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHFR antibody detected the CHFR protein (arrow).)

Related Product Information for anti-CHFR antibody

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Product Categories/Family for anti-CHFR antibody

Antibodies; Ubiquitin Ligases

References

Soutto, M., et al. Cancer 116(17):4033-4042(2010). Kim, J.M., et al. Biochem. Biophys. Res. Commun. 395(4):515-520(2010). Hiraki, M., et al. World J. Gastroenterol. 16(3):330-338(2010). Baba, S., et al. Oncol. Rep. 22(5):1173-1179(2009). Gao, Y., et al. Int. J. Biol. Markers 24(2):83-89(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 239048905
• NCBI GeneID: 55743
• NCBI Accession #: NP_001154818.1; NP_001154817.1
• NCBI GenBank Nucleotide #: NP_001154818.1
• UniProt Accession #: Q96EP1
• NCBI Official Full Name: E3 ubiquitin-protein ligase CHFR isoform 3
• NCBI Official Synonym Full Names: checkpoint with forkhead and ring finger domains
• NCBI Official Symbol: CHFR
• NCBI Official Synonym Symbols: RNF116; RNF196
• NCBI Protein Information: E3 ubiquitin-protein ligase CHFR
• UniProt Protein Name: E3 ubiquitin-protein ligase CHFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: CHFR
• UniProt Synonym Gene Names: RNF196
• UniProt Entry Name: CHFR_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]

Uniprot Description

CHFR: E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. Interacts with HDAC1 and HDAC2. Interacts with PML (with sumoylated form of PML). Ubiquitous. Belongs to the CHFR family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: PML body; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; nucleotide binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; modification-dependent protein catabolic process; mitosis; protein polyubiquitination; cell division; mitotic cell cycle checkpoint