Rabbit CHD4 Polyclonal Antibody | anti-CHD4 antibody

Anti-CHD4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101868

• Gene Names: CHD4; Mi-2b; Mi2-BETA
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: CHD4; Polyclonal Antibody; Anti-CHD4 Antibody; Rabbit Polyclonal; CHD4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-Mi-2b Antibody; Anti-Mi2-BETA Antibody; anti-CHD4 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CHD4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CHD4 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human CHD4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CHD4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CHD4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CHD4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.)

Related Product Information for anti-CHD4 antibody

Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATPase that alters the phasing of nucleosomes on DNA and has recently been implicated in DNA double-stranded break (DSB) repair. CHD4 depletion renders AML blasts more sensitive both in vitro and in vivo to genotoxic agents used in clinical therapy: daunorubicin (DNR) and cytarabine (ara-C). CHD4 is a novel therapeutic target whose inhibition has the potential to enhance the effectiveness of genotoxic agents used in AML therapy. Chromodomain Helicase DNA-binding protein 4 (CHD4) is a chromatin-remodeling enzyme that has been reported to regulate DNA-damage responses through its N-terminal region in a poly(ADP-ribose) polymerase-dependent manner. The chromatin-remodeling enzyme CHD4 maintains vascular integrity at mid-gestation. CHD4-deficient embryos developed normal lymphovenous (LV) valves, which regulate the return of lymph to the blood circulation; however, these valves lacked the fibrin-rich thrombi that prevent blood from entering the lymphatic system. Chromodomain helicase DNA-binding protein 4 (CHD4) is an ATP-dependent chromatin remodeler involved in epigenetic regulation of gene transcription, DNA repair, and cell cycle progression. Also known as Mi2beta, CHD4 is an integral subunit of a well-characterized histone deacetylase complex.

NCBI and Uniprot Product Information

• NCBI GI #: 51599156
• NCBI GeneID: 1108
• NCBI Accession #: NP_001264.2
• NCBI GenBank Nucleotide #: NM_001273.2
• UniProt Accession #: Q14839; Q8IXZ5
• Molecular Weight: 218,005 Da
• NCBI Official Full Name: chromodomain-helicase-DNA-binding protein 4
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 4
• NCBI Official Symbol: CHD4
• NCBI Official Synonym Symbols: Mi-2b; Mi2-BETA
• NCBI Protein Information: chromodomain-helicase-DNA-binding protein 4; CHD-4; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein
• UniProt Protein Name: Chromodomain-helicase-DNA-binding protein 4
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: CHD4
• UniProt Synonym Gene Names: CHD-4
• UniProt Entry Name: CHD4_HUMAN

NCBI Description

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. [provided by RefSeq, Jul 2008]

Uniprot Description

CHD-4: is an ATP-dependent helicase and a central component of the HDAC-containing nucleosome remodeling and histone deacetylase (NuRD) transcriptional complex. Interacts with MTA1/2, HDAC1, and RbAp46 in the NuRD complex. CHD-4 belongs to the SNF2/RAD54 helicase family of proteins. The NuRD complex, whose subunit composition varies by cell type and in response to physiologic signals, plays central roles in many processes including oncogenesis and metastasis, B cell differentiation, invasive growth of breast cancer, and maintaining the balance between the undifferentiated state and the process of differentiation in embryonic stem cells.

Protein type: Helicase; DNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nucleoplasm; centrosome; protein complex; membrane; nuclear chromatin; cytoplasm; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; nucleosomal DNA binding; DNA binding; zinc ion binding; microtubule binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; ATP-dependent chromatin remodeling; spindle assembly; DNA duplex unwinding